Study of white frangipani flower and bitter grape stem ethanol extract combination on antibacterial and antioxidant activities

KRISHNA PURNAWAN CANDRA, WIJAYANTI KUSUMA WARDHANI, ANTON RAHMADI, MIFTAKHUR ROHMAH, YULIANI YULIANI

Abstract


Frangipani flower (Plumeria acuminata) and bitter grape (Tinospora crispa L. Miers) stem extract alone shows antimicrobial and antioxidant activities. However, there is a limited report about the actions from the combination of both extracts. Combinations of ethanol extract of white frangipani flower (EFF) and ethanol extract of the bitter grape stem (EBS), each of 0, 1, 2, and 3% were tested for inhibition against Staphylococcus aureus growth and DPPH (1,1-diphenyl,2-picrylhydrazyl) free radicals. A yield of 3.17 and 2.62 g of EFF and EBS, respectively, were resulted from 100 g of dried powdered of the white frangipani flower and bitter grape stem using 200 mL of absolute ethanol. The antimicrobial test was assayed using a 10% stock solution of extract diluted in water. The combination of EFF and EBS showed a synergistic effect on antimicrobial activity, but it proved an antagonistic effect on antioxidant activity. The combination of EFF 3% and EBS 3% showed the highest inhibition index on the S. aureus growth , i.e. 2.02±0.06, which is higher than the inhibition index of Amoxycillin 2%, i.e. 1.85±0.09. The highest antioxidant activity showed by 1% EFF alone (57.5 ± 0.60%), while the lowest was from the combination of EFF 1% and EBS 3% (23.4±0.30%).

 

 


Keywords


Plumeria, bitter grape, Tinospora, Staphylococcus aureus

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References


Shinde, P. R.; Patil P. S.; Bairagi, V. A. 2014. Phytopharmacological Review of Plumeria species. Scholars Academic Journal of Pharmacy 3(2) 2320–4206. Retrieved from www.saspublisher.com

Choudhary, M.; Kumar, V.; Singh, S. 2014. Phytochemical and pharmacological activity of Genus Plumeria: An update review. Imternational Journal of Biomedical and Advance Research 5(6) 266–271. https://doi.org/10.7439/ijbar

Farooque, A.; Mazumder, A.; Shambhawee, S.; Mazumder, R. 2012. Review on Plumeria acuminata. International Journal of Research in Pharmacy and Chemistry 2(2) 467–469.

Husni, M. A.; Murniana; Helwati, H.; Nuraini. 1993. Antimicrobial activity of n-hexane extracts of red frangipani (Plumeria rocea). Jurnal Natural 13(1) 28–33.

Syakira, M. H.; Brenda, L. 2010. Antibacterial capacity of Plumeria alba petals. International Scholarly and Scientific Research & Innovation 4(8) 1202–1205.

Jiwantono, F.; Purwanta, M.; Setiawati, Y. 2017. Uji efektifitas ekstrak bunga kamboja (Plumeria alba) sebagai antibakteri terhadap Streptococcus pyogenes [Effectivity test on frangipani flower extract (Plumeria alba) as antibacterial on Streptococcus pyogenes]. Jurnal Kedokteran Syiah Kuala 17(3) 147–155. https://doi.org/10.24815/jks.v17i3.9066

Maulani, A. 2013. Pengaruh daya antibakteri ekstrak etanolik bunga kamboja putih (Plumeria alba) terhadap pertumbuhan bakteri plak gigi Aggregatibacter actinomycetemcomitans (Kajian secara in vitro) [Effect on antibacterial capacity of ethanol extract (Plumeria alba) on the thoot plaque bacteria (Aggregatibacter actinomyce-temcomitans (in vitro study). Thesis. Dentristry Department. (Yogyakarta: Universitas Gadjah Mada).

Wrasiati, L. P.; Hartati, A.; Yuarini, D. A. A. 2011. Kandungan senyawa bioaktif dan karakteristik sensoris ekstrak simplisia bunga kamboja (Plumeria sp.) [Bioactive compounds and sensory characteristics of simplisia extract of frangipani (Plumeria sp.)]. Jurnal Biologi 15(2) 39–43.

Mohammed, A. I. C.; Gunjan, M.; Dinesh, C. K. 2012. Antimicrobial activity of Tinospora crispa root extracts. International Journal of Research in Ayurveda and Pharmacy 3(3) 417–419.

Sulaiman, F. A.; Fuad, N.; Rahman, F.; Iqbal, A.; Darnis, D. S. 2016. Antioxidant and antimicrobial properties of Tinospora crispa (putarwali) stems methanolic extract. Jurnal Teknologi 78(11–2) 35–40. https://doi.org/10.11113/jt.v78.9941

Spinella, M. 2002. The importance of pharmacological synergy in psychoactive herbal medicines. Alternative Medicine Review 7(2) 130–137.

Ciesla, L. M.; Wojtunik-Kulesza, K. A.; Oniszczuk, A.; Waksmundzka-Hajnos, M. 2016. Antioxidant synergism and antagonism between selected monoterpenes using the 2,2-diphenyl-1-picrylhydrazyl method. Flavour and Fragrance Journal 31(6) 412–419. https://doi.org/10.1002/ffj.3330

Sonam, K. S.; Guleria, S. 2017. Annals of pharmacology and pharmaceutics synergistic antioxidant activity of natural products. Annals of Pharmacology and Pharmaceutics 2(8) 1086.

Adwan, G.; Mhanna, M. 2008. Synergistic effects of plant extracts and antibiotics on Staphylococcus aureus strains isolated from clinical specimens. Middle-East Journal of Scientific Research 3(3) 134–139.

Farhan, H.; Rammal, H.; Hijazi, A.; Hamad, H.; Daher, A.; Reda, M.; Badran, B. 2012. In vitro antioxidant activity of ethanolic and aqueous extracts from crude Malva parviflora L. grown in Lebanon. Asian Journal of Pharmaceutical and Clinical Research 5(SUPPL. 3) 234–238.

Furtado, G. L.; Medeiros, A. A. 1980. Single-disk diffusion testing (Kirby-Bauer) of susceptibility of Proteus mirabilis to chloramphenicol: Significance of the intermediate category. Journal of Clinical Microbiology 12(4) 550–553.

Prihardini; Kristianingsih, I. 2016. Uji aktivitas antibakteri minyak atsiri dan ekstrak etanol bunga kamboja putih (Plumeria acuminata L.) terhadap Escherichia coli [Antibacterial activity test of essential oil of white frangipani flower (Plumeria acuminata L.) on Escherichia coli]. In Prosiding Seminar Nasional Tumbuhan Obat Indonesia ke-50 (pp. 215–223). (Samarinda: Fakultas Farmasi Universitas Mulawarman).

Megawati; Saputra, S. W. D. 2012. Miyak atsiri dari kamboja kuning, putih, dan merah dari ekstraksi dengan n-heksana [Essential oil from n-hexane extract of yellow, white and red frangipani]. Jurnal Bahan Alam Terbarukan 1(1) 25–31.

Irianti, T.; Puspitasari, A.; Suryani, E. 2011. Aktivitas penangkapan radikal 2,2-difenil-1-pikrilhidrazil oleh ekstrak etanolik (T. crispa (L.) Miers) dan fraksi-fraksinya. Majalah Obat Tradisional 16(3) 139–146.

Yulianingtyas, A.; Kusmartono, B. 2016. Optimasi volume pelarut dan waktu maserasi pengambilan flavonoid daun belimbing wuluh (Averrhoa bilimbi L.) [Optimization of solvent volume and time of maceration extraction of flavonoid from mini starfruit (Averhoa bilimbi L.)]. Jurnal Teknik Kimia 10(2) 58–64. https://doi.org/10.1016/j.annemergmed.2013.08.024

Zakaria, Z. A.; Zakaria, M. L.; Amon, Z.; Desa, M. N. M. 2011. Antimicrobial activity of the aqueous extract of selected Malaysian herbs. African Journal of Microbiology Research 5(30) 5379–5383. https://doi.org/10.5897/ajmr11.874

Esimone, C. O.; Iroha, I. R.; Ibezim, E. C.; Okeh, C. O.; Okpana, E. M. 2006. In vitro evaluation of the interaction between tea extracts and penicillin G against Staphylococcus aureus. African Journal of Biotechnology 5(11) 1082–1086.




DOI: https://doi.org/10.24815/jn.v20i3.17175

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