Pengaruh Bakteriosin Produksi Bakteri Asam Laktat Isolat Indonesia terhadap Jumlah Bakteri dalam Susu Pasteurisasi

Nurliana Nurliana, Idwan Sudirman, Mirnawati Sudarwanto, Raden Roso Soejoedono


The effect of bacteriocin produced by Indonesia Isolate of lactic acid bacteria toward total bacteria in pasteurized milk

ABSTRACT. The aim of this research was to study the ability of bacteriocin (amylocin) produced by Indonesian isolate of Lactic Acid Bacteria (LAB) (Lactobacillus amylovorus US 121) and its combination with nisin in pasteurized milk. The total bacteria was measured by plate count method. The antimicrobial activity of amylosin at 64 AU/ml had the same activity with nisin at 625 AU/ml and its combination between amylosin and nisin at 32 AU/ml and 312,5 AU/ml respectively against psychrotrophic, thermoduric bacteria and L. monocytogenes in pasteurized milk. However, combination of amylosin and nisin (32 AU/ml + 312,5 AU/ml) able to reduce S. aureus in pasteurized milk. Amylosin at 64 AU/ml kept the shelf life of pasteurized milk longer until 12 days at 10oC. The addition of amylosin (the precipitate resulted by ammonium sulfate precipitation at 70% w/v) changed the color of pasteurized milk be brownish white.


bacteriocin; amylopsin; pasteurized milk

Full Text:



Barefoot, S.F., Nettles. C.G., 1993. Antibiotics Revisited : Bacterious Produced by Dairy Starter Cultures. J. Dairy Sci. 76 : 2366-2379.

Buchanan, R.L. and Klawitter, L.A., 1992a. Effectiveness of Carnobacterium piscicola LK5 for Controlling the Growth of Listeria monocytogenes Scott A in Refrigerated Foods. J. Food Safety. 12: 219-236.

Buchanan, R.L. and Klawitter, L.A., 1992b. Characterization of a Lactic Acid Bacterium, Carnobacterium piscicola LK5, with Activity Against Listeria monocytogenes at Refrigeration Temperatures. J. Food Safety. 12: 199-217.

El-Khateib, T., Yousef, A.E. and Ockerman, H.W., 1993. Inactivation and Attachment of Listeria monocytogenes on Beef Muscle Treated with Lactic Acid and Selected Bacteriocins. J. Food Protection. (56) 1 : 29-33.

Fox, B.A. and Cameron, A.G., 1989. Food Science, Nutrition and Health. 5th ed. Edward Arnold, London.

Hanlin, M.B., Kalchayanand, N., Ray, P. and Ray, B., 1993. Bacteriocins of Lactic Acid Bacteria in Combination Have Greater Antibacterial Activity. J. Food Protection. 56: 252-255.

Harris, E.L.V., 1989. Concentration of The Extract. In: Methods a Practical Approach. Oxford University Press, Oxford.

Hobbs, B.C. and Roberts, D., 1997. Food Poisoning and Food Hygiene. 5th ed. Edward Arnold, London.

Holzapfel, W.H., Geisen, R. and Schillinger, U., 1995. Biological Preservation of Foods with Reference to Protective Cultures, Bacteriocins and Food-Grade enzymes. Int. J. Food Microbiol. 24: 343-362.

Liao, Chii-Cherng, Yousef, A.E., Chism, G.W. and Ritcher, E.R., 1994. Inhibition of Staphylococcus aureus in Buffer, Culture Media and Foods by Lacidin A, a. Bacteriocin Produced by Lactobacillus acidophillus OSU 133. J. Food Safety. 14: 87-101.

Marugg, J.D., 1991. Bacteriocins, Their Role in Developing Natural Products. Food Biotechnology. 5: 305-312.

Masnier-Patin, S., Deschamps, N., Tatini, S.R. and Richard, J., 1992. Inhibition of L. monocytogenes in Camembert Cheese made with a Nisin-Producing Starter. Lait, 72: 249-263.

Monticello, D.J. and O’Connor, D., 1990. Lysis of L .monocytogenes by Nisin, Chapter 14: 18-83. In: Foodborne Listeriosis.

Motlagh, A.M., Johnson, M.C. and Ray, B., 1991. Viability Loss of Foodborne Pathogenes by Starter Culture Metabolites. J Food Protection. 54: 873-878, 884.

Motlagh, A. M., Holla, S., Johnson, M.C., Ray, B. and Field, R.A., 1992. Inhibition of Listeria.spp in Sterile Food System by Pediocin AcH, A Bacteriocin Produced by Pediococcus acidilactic H. J. Food Protection. 55: 337-343.

Mustafa, Z., 1990. Microstat untuk Mengolah Data Statistik. Andi Offset, Yogyakarta.

Nettles, C.G. and Barefoot, S.F., 1993. Biochemical and Genetic Characteristic of Bacteriocins of Food Associated Lactic Acid Bacteria. J. Food Protection. 56: 338-356.

Ray, B., 1992. Nisin of Lactobacillus lactis ssp Lactis as a Food Biopreservative, 207-264. In: B. Ray and M. Daeschel (ed). Food Biopreservatives of Microbial Origin. CRC Press. Boca Raton.

Rozbeh, M., Kalchayanand, N., Field, R.A., Johnson, M.C. and Ray, B., 1993. The Influence of Biopreservatives on the Bacterial Level of Refrigerated Vacuum Package Beef. J. Food Safety. 13 : 99-111.

Schillinger, U. and Lucke, F.K., 1989. Antibacterial Activity of Lb. sake Isolated from Meat. Appl. Environ. Microbiol. 8:1901-1906.

Schillinger, U. and Lucke., F.K., 1991. Behavioral of L. monocytogenes in Meat and Its Control by a Bacteriocin-Producing Strain of Lb. sake. J. Appl. Bacteriology. 70: 473-478 .

Schillinger, U. Becker, B. and Holzapfel, W.H., 1995. Antilisterial Activity of Carnocin 54, a by Bacteriocin from Leuconostoc carnosum. Food Microbiol. 12: 31-37.

Sofos, J. N., 1993. Current Microbiological Consideration in Food Preservation. Int. J. Food Microbiol. 19: 87-108.

Stiles, M.E. and Hastings, J.W., 1991. Bacteriocin Production by Lactic Acid Bacteria: Potential for Use in Meat Preservation. Review. Trends in Food Science and Technology. 10: 247-251.

Sudarwanto, M., 1996. Komposisi dan Pembentukan Susu. Kursus Singkat Jaminan Mutu dalam Industri Susu. Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Bogor.

Sudirman, I., Mathieu, F., Michael, M. and Lefebvre, G., 1993. Detection and Properties of Curvaticin 13, a Bacteriocin-Like Substance Produced by Lactobacillus curvatus SB 13. Current Microbiol. 27: 35-40

Swanson, K.M.J., Busta,F.F., Peterson, E.H. and Johnson, M.G., 1992. Colony Count Methods. In: C. Vanderzant and D. F. Splittstoesser (ed). Compendium of Methods for The Microbiological Examination of Foods. 3rd ed. American Public Health, Washington.

Winkowski, K. and Montville, T.J., 1992. Use of Meat Isolate, Lactobacillus bavaricus MN to Inhibit Listeria monocytogenes Growth in Model Meat Gravy System. J. Food Safety 13: 19-31.



  • There are currently no refbacks.

Copyright (c)

Creative Commons LicenseISSN: 1411-4623E-ISSN: 2460-4534
Copyright© 2000-2020 | ISSN: 1411-4623 | EISSN: 2460-4534 
Jurnal Agripet is licensed under a Creative Commons Attribution 4.0 International License.


Published by: 
Animal Husbandry DepartmentThe Faculty of Agriculture, Syiah Kuala University 
associated with Animal Scientist's Society of Indonesia (HILPI)
Jl. Tgk. Hasan Krueng Kalee No. 3, Kopelma Darussalam,
Banda Aceh, 23111, Indonesia.
Phone: +62-81383736633
Email: &

Online Submissions & Guidelines Editorial Policies | Contact Statistics Indexing | Citations