Kajian Titer Antibodi Pada Yolk dari Ayam yang Diimunisasi Dengan Antigen Ekskretori/Sekretori Stadium L3 Ascaridia galli

Darmawi Darmawi, Ummu Balqis, Risa Tiuria, Muhammad Hambal, Samadi Samadi


Evaluation of antibody titre in yolk from immunized chickens with excretory/secretory antigen of L3 stage of Ascaridia galli

ABSTRACT. The purpose of the present study was to trigger humoral immunity of chickens egg yolk exposed to excretory/secretory released in vitro by L3 stage of Ascaridia galli. Amount of 6 head chickens were divided into two groups. First group, the chickens were not immunized. Second group, the chickens were immunized with excretory/ secretory. Active immunizations with excretory/ secretory antigen were applied intra muscularly of chickens with an initial dose of 80 μg. The immunizations were repeated three times with dose of each 60 μg with an interval of one week. The first immunizations were excretory/secretory antigen mixed with Fruend Adjuvant Complete and subsequently mixed with Freund Adjuvant Incomplete. Antibody response in yolk was detected at weekly intervals by enzyme linked immunosorbant assay (ELISA). The result showed that antibody in yolk was begun detect with ELISA increased at two weeks after immunization, This study has shown that the excretory/secretory released in vitro by L3 stage A. galli is capable of stimulating humoral immunity by mean of producing IgY in yolk.


Ascaridia ; excretory antigen; secretory antigen; antibody

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Camenisch, G., Tini, M., Chilov, D., Kvietikova, I., Srinivas, V., Caro, J., Spielmann, P., Wenger, R. H. and Gassmann, M., 1999. General Applicability of Chicken Egg Yolk Antibodies: the Performance of IgY Immunoglobulins Raised Against the Hypoxia-inducible Factor 1. J. FASEB. 13: 81-88.

Carlander, D., 2002. Avian IgY Antibody: in vitro and in vivo. Dissertation, Acta Universitatis Upsaliensis, Uppsala.

Green, RS., 1996. Antibody Responses of Grazing Alpacas (Lama pacos) in New Zealand to Intestinal Nematodes. Int. J. for Parasitol. 26(4): 429 – 435.

Haak-Frendscho, M., 1994. Why IgY? Chicken Polyclonal Antibody, an Appealing Alternative. Promega Notes Magazine (46): 11.

Hanly, W.C., Artwohl, J.E. and Bennett, B.T., 1995. Review of Polyclonal Antibody Production Procedures in Mammals and Poultry. ILAR News 37:93.

Harnett, W., MacDonald, M., Preece, G., Patterson, M. and Parkhouse, M.E., 1997. Production of Monoclonal Antibodies Against Excretory-Secretory Products of Adult Male Onchocerca gibsoni. J. of Parasitol. 83(2): 316 – 319.

Hintz, M., Schares, G., Taubert, A., Geyer R., Zahner, H., Stirm, S. and Conraths, F.J., 1998. Juvenile Female Listomosoides sigmodontis Produce an Excretory-Secretory Antigen (Juv-p120) Highly Modified with Dimethylaminoe-thanol. J. of Parasitol. 171: 265-271.

Leenaars, M. and Hendriksen, C.F.M., 2005. Critical Steps in the Production of Polyclonal and Monoclonal Antibodies: Evaluation and Recommendations, ILAR J. 46: 269 - 279.

McKeand, J.B., Knox, D.P., Duncan, J.L. and Kennedy, M.W., 1995. Protective Immunization of Guinea Pigs Against Dictyocaulus viviparus Using Excretory/Sexcretory Product of Adult Parasites. Int. J. for Parasitol. 25: 93 – 104.

Mine, Y. and Kovacs-Nolan, J., 2002. Chicken Egg Yolk Antibodies as Therapeutics in Enteric Infectious Disease: A Review. J. Med. Food 5: 159 – 169.

Nagano, 2004. Molecular Expression of a Cysteine Proteinase of Clonorchis sinensis and Its Application to an Enzyme-Linked Immunosorbent Assay for Immunodiagnosis of Clonorchiasis. Clinical and Diagnostic Laboratory Immunology, Am. for Microbiol. 11(2): 411 - 416 http://cdli.asm.org/cgi/content/ full/11/2/411 (01-03-2005)

Roitt, I.M. and Delves, P.J., 2001. Roitt’s Esential Immunology. Tenth Edition, Blackwell Science Ltd. Osney Mead Oxford OX2 OEL.

Rollier, C., Charollois, C., Jamrd, C., Trepo, C. and Cova, L., 2000. Maternally Transferred Antibodies from DNA-Immunized Avians Protect Offspring Against Hepadnavirus Infection. J. of Virol. 74(10): 4908 – 4911.

Schade, R., Henklein, P. and Hlinak, A., 1999. The Production of Avian (Egg Yolk) Antibodies: IgY. The Report And Recommendations of ECVAM Workshop 211,2. Reprinted with Minor Amend-ments from ATLA 24: 925 - 934.

Schmidt, P., Wiedemann, V., Kühlmann, R., Wanke, R., Linckh, E. and Lösch, U., 1989. Production of Antibodies to Canine Distemper Virus in Chicken Eggs for Immunochemistry. J. of Vet. Med. B 36: 661 – 668.

Soejoedono, R.D., Wibawan, I.W.T. dan Hayati, Z., 2005. Pemanfaatan Telur Ayam Sebagai Pabrik Biologis: Produksi ”Yolk Immunoglobulin” (IgY) Anti Plaque dan Diare dengan Titik Berat pada Anti Streptococcus mutans, Escherichia coli dan Salmonella enterotidis. Laporan Riset Unggulan Terpadu, Kementerian Negara Riset dan Teknologi Republik Indonesia.

Szabo, C.S., Bardos, L., Losonczy, S. and Kachesz, K., 1998. Isolation of Antibodies from Chicken and Quail Eggs. Presented at INABIS ’98 – 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, December 7–16th. http://www.mcmaster.ca/inabis98/immunology/-szabo0509/index.html (20-11-06)

Tiuria, R., Ridwan, Y. and Murtini, S., 2003. Study of Bioactive Substance from Ascaridia galli Adult Worm that Stimulate Intestinal Mucosal Defense Mechanism in Chicken for Medical Purpose. Proceeding of the Seminar on Science and Technology, Indonesia-Toray Science Foundation, Jakarta.

Yadav, S.C, Saini, M., Raina, O.K., Nambi, P.A., Jadav, K. and Sriveny, D., 2005. Fasciola gigantica Cathepsin-L Cysteine Proteinase in the Detection of Early Experimental Fasciolosis in Ruminants. http://parasitology.informatik.uni-wuerzburg.de/login/n/h/j_436_005-1466-8.html.html (21-02-2006) Yoshihara, S., Oya, T., Furuya, T. and Goto, N., 1993. Use of Body Fluid of Adult Female Ascaris suum as an Antigen in the Enzyme-Linked Immu-nosorbent Assay (ELISA) for Diagnosis of Swine Ascariosis. J. of Helminthol. 67: 279 – 286.

DOI: https://doi.org/10.17969/agripet.v8i2.611


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