Hubungan Polimorfisme Gen Laktoferin dengan Kualitas Susu pada Sapi Perah Friesian-Holstein

Elmy Mariana


Association of Lactoferrin Polymhorphism on Milk Yield and Milk Quality in Holstein-Friesian Dairy Cows

ABSTRACT. The purposes of this study were to identify the polymorphism of the lactoferrin gene and the association of genetic variants of the lactoferrin gene on milk quality in Holstein-Friesian (HF) cows. The study was conducted on 194 heads of HF lactating cows coming from semi intensive of small dairy farmers in two villages of the KPSBU Lembang, Lembang district. Investigation on variant genotypes of the lactoferrin gene used PCR-RFLP method. Genotyping of the lactoferrin gene with EcoRI restriction enzyme produced two genotypes, i.e. AA (65%) and AB (35%) genotypes. Both the AA and AB genotypes had inconsistency in controlling component of the milk quality. Further, the AB lactoferrin cows had a higher frequency in the classification of low somatic cells count (0.75), while the AA cows were at a higher frequency in the classification of high somatic cells count (0.60). It was concluded that there is no strong relationship between intron 6 lactoferrin gene with milk quality parameters. Further, the AB genotype of the lactoferrin gene had a consistency in producing lower somatic cells count compared to the AA genotype.


polymorphism; lactoferrin gene; milk quality; PCR-RFLP

Full Text:



Ashwell, M. S., Heyen, D. W., Sonstegard, T. S., Van Tassel, C. P., Da, Y., Van, P. M., Raden, Ron, M., Weller, J. I., and Lewin, H. A., 2004. Detection of quantitative trait loci affecting milk production, health, and reproduction traits in Holstein cattle. J. Dairy Sci. 87:468-475.

Arnould, V. M. R., Soyeurt, H., Gengler, N., Colinet, F. G., Georges, M. V., Bertozzi, C., Portetelle, D., and Renaville, R., 2009. Genetic analysis of lactoferrin content in bovine milk. J. Dairy Sci. 92:2151-2158.

Boichard, D., Grohs, C., Bourgeois, F., Cerqueira, F., Faugeras, R., Neau, A., Rupp, R., Amigues, Y., Boscher, M. Y., and Levéziel, H., 2003. Detection of genes influencing economic traits in three French dairy cattle breeds. Genet. Sel. Evol. 35:77-101.

Connely, O. M., 2001. Antiinflammatory activities of lactoferrin. J. Am. Coll. Nutr. 20(5):389-395s.

Dewan Standarisasi Nasional. 1998. SNI 01-2782.1998. Susu Segar. Dewan Standarisasi Nasional, Jakarta.

Hagiwara, S. I., Kawai, K., Anri, A., and Nagahata, H., 2003. Lactoferrin concentrations in milk from normal and subclinical mastitic cows. J. Vet. Med. Sci. 65:319-322.

Harder, B., Bennewitz, J., Reinsch, N., Thaller, G., Thomsen, H., Kühn, C., Schwerin, M., Erhardt, G., Förster, M., Reinhardt, F., and Kalm, E, 2006. Mapping of quantitative trait loci for lactation persistency traits in German Holstein dairy cattle. J. Anim. Breed. Genet. 123:89-96.

Kaminski S., Malewski, T., Ahman, A., Wójcik, E., Rusc, A., Olenski, K., Jakubczak, A., and Sazanov, A. A., 2008. Towards an integrated approach to study SNPs and expression of candidate genes associated with milk protein biosynthesis. Russian J. Genet. 44:459-465.

Kaminski S., Olenski, K., Brym, P., Malewski, T., and Sazanov, A. A., 2006. Single nucleotide polymorphism in the promoter region of the lactoferrin gene and its associations with milk performance traits in Polish Holstein-Friesian cows. Russian J. Genet. 42:924-927.

Klussmann, U., and Seyfert, H. M., 1995. Genetische Varianten des bovinen Laktoferrins, einem Kandidatengen fur Mastitisresistenz. Vortragtagung der DGfZ/GfT, Hannover.

Li, G. H., Zhang, Y., Sun, D. X., and Li, N., 2004. Study on the polymorphism of bovine lactoferrin gene and its relationship with mastitis. Anim. Biotech. 15:67-76.

Mattjik, A. A., dan Sumertajaya, M., 2006. Perancangan Percobaan dengan Aplikasi SAS dan Minitab, Jilid 1. IPB Press, Bogor.

Mrode, R. A., Swanson, G. J. T., and Winters, M. S., 1998. Genetic parameters and evaluations for somatic cell count and its relationship with production and type traits in some dairy breeds in the Uinted Kingdoms. Anim. Sci. 66:569-576.

Nei, M., 1987. Molecular Evalutionery Genetics. Columbia University Press, New York.

Nibbering, P. H. E., Ravensbergen, M. M., Welling, L. A., van Berkel, P., van Berkel, H., Pauwels, K., and Nuijens, J. H., 2001. Human lactoferrin and peptides derived from its N terminus are highly effective against infections with antibiotic-resistant bacteria. Infect. Immun. 69 :1469-1476.

Ng-Kwai-Hang, K. F., Monardes, H. G., and Hayes, J. F., 1990. Association between genetic polymorphism of milk proteins and production traits during three lactations. J. Dairy Sci. 73:3414-3420.

Pawlik, A, Sender, G., and Kossakowska, A. K., 2009. Bovine lactoferrin gene polymorphism and expression in relation to mastitis resistance – a review. Anim. Sci. Papp. Reprod. 27:263-271.

Rainard, P., and Riollet, C., 2006. Innate immunity of the bovine mammary gland. Vet. Res. 37:369-400.

Sambrok, J., Fritsch, F., and Miniatis, T., 1989. Molecular Clooning Laboratory Manual. 3nd ed. Cold Spring Harbor Laboratory Press, New York.

Schanbacher, F.L., Goodman, R. E., and Tallhouk, R. S., 1993. Bovine mammary lactoferrin : implication from messenger ribonucleic acid (mRNA) sequence and regulation contrary to other milk proteins. J. Dairy. Sci. 76:3812-3831.

Schukken, Y.H., Wilson, D. J., Welcome, F., Garrison-Tikofsky, L., and Gonzalez, R. N., 2003. Monitoring udder health and milk quality using somatic cell counts. Vet. Res. 34: 579-596.

Sender, G., Korwin-Kossakowska, A., Galal, K., and Prusak B., 2006. Ocena wplywu polimorfizmu wybranych genów na wystepowanie mastitis u krów in polish. Med. Wet. 62:563-565.

Seyfert, H. M., Henke, M., Interthal, H., Klussmann, H., Koczan, D., Natour, S., Pusch, W., Senft, B., Steinhoff, U. M., Tuckoricz, A., and Hobom. G., 1996. Defining candidate genes for mastitis resistance in cattle: The role of lactoferrin and lysozyme. J. Anim. Breed. Genet. 113:269-276.

Seyfert, H. M. and Kuhn, C., 1994. Characterization of a first bovine lactoferrin gene variant, based on an EcoRI polymorphism. Anim. Gen. 25:54.

Seyfert, H. M., Tuckoricz, A., Intertahl, H., Koczan, D., and Hobom, G., 1994. Structure of the bovine lactoferrin-encoding gene and its promoter. Anim. Genet. 143:265-269.

Wakabayashi, H., Yamauchi, K., and Takase, M., 2006. Lactoferrin research, technology and applications. Int. Dairy. J. 6:1241-1251.

Wojdak-Maksymiec, K., Kmiec, M., and Ziemak, J., 2006. Associations between bovine lactoferrin gene polymorphism and somatic cell count in milk. Vet. Med. 51:14-20.

Zhao, C. H., He, G. M., Wang, Y. L., and Zhang, Z. X., 2008. Polymorphism analysis of the promoter of cow lactoferrin gene with PCR-RFLP and its correlation with subclinical mastitis. Acta Agricultur. Slovea. 92:185-187.



  • There are currently no refbacks.

Copyright (c)

Creative Commons LicenseISSN: 1411-4623E-ISSN: 2460-4534
Copyright© 2000-2020 | ISSN: 1411-4623 | EISSN: 2460-4534 
Jurnal Agripet is licensed under a Creative Commons Attribution 4.0 International License.


Published by: 
Animal Husbandry DepartmentThe Faculty of Agriculture, Syiah Kuala University 
associated with Animal Scientist's Society of Indonesia (HILPI)
Jl. Tgk. Hasan Krueng Kalee No. 3, Kopelma Darussalam,
Banda Aceh, 23111, Indonesia.
Phone: +62-81383736633
Email: &

Online Submissions & Guidelines Editorial Policies | Contact Statistics Indexing | Citations