Populasi Ascaridia galli Dalam Usus Halus Ayam Yang Diberikan Kombinasi Ekskretori/Sekretori L3 dan Imunoglobulin Yolk

Darmawi Darmawi, Ummu Balqis, Risa Tiuria

Abstract


Ascaridia galli populations in intestine of chickens treated with combination of excretory/secretory L3 and immunoglobulin yolk

ABSTRACT. The purpose of the present study was to determine the presence of worm populations in intestine of chickens vaccinated and combined with egg yolk to experimental Ascaridia galli infection. Amount of 18 head chickens were devided into six groups (A – F). Group A, the chickens were not vaccinated. Group B, the chickens were vaccinated with excretory/secretory of A. galli L3. Group C, the chickens were vaccinated with excretory/secretory of A. galli L3, challenged with dose 1000 L2, and treated ten times with 0,875 mg egg yolk with an interval of one day intra orally. Group D, the chickens were vaccinated with excretory/secretory of A. galli L3 and challenged with dose 1000 L2. Group E, the chickens were challenged with dose 1000 L2 and treated ten times with 0,875 mg egg yolk with an interval of one day intra orally. Group E, the chickens were challenged with dose 1000 L2. Intestinal worm burdens of infected groups were recorded. The result showed that excretory/secretory of A. galli L3 combined with egg yolk decreased significantly A. galli survival in intestine of laying hens. Vaccinations were positively correlated with worm burden at 12 weeks after chalanged. The results suggest that A. galli L3 excretory/secretory product contain potential antigen and that antibody-mediated mechanisms contribute to immune protection.


Keywords


ascaridia galli; excretory; secretory; yolk immunoglobulin

Full Text:

PDF

References


Akita, E. M., and Nakai, S., 1992. Immunoglobulins from egg yolk: isolation and purification. J. of Food Sci. 57:629-634.

Abe, T., Nawa, Y., and Yoshimura, K., 1992. Protease resistant interleukin-3 stimulating components in excretory and secretory products from adult worms of Strongyloides ratti. J. of Helminthol. 66:155-158.

Camenisch, G., Tini, M., Chilov, D., Kvietikova, I., Srinivas, V., Caro, J., Spielmann, P., Wenger, R. H., and Gassmann, M., 1999. General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobulins raised against the hypoxia-inducible factor. 1J. FASEB. 13:81-88.

Carlander, D., 2002. Avian IgY Antibody: in vitro and in vivo. Dissertation, Acta Universitatis Upsaliensis, Uppsala.

Dahl, C., 2002. The Effect of Concurrent Infections With Pasteurella multocida and Ascaridia galli on Free Range Chickens. Vet. Microbiol. 86:313-324. http://orgprints.org/00001857. [21 Mei 06]

Darmawi, Balqis, U., Tiuria R., Suhartono M.T., Soejoedono, R.D., Priosoeryanto, B.P., Pasaribu, F. H., and Hambal, M., 2006. Protease Activity of Excretory/Secretory Released by Invasive Stage of Ascaridia galli. In : Procceding Enzymes: Industrial and Medical Prospects, ASEAN Biochemistry Seminar, Surabaya. 6-7th February.

Darmawi, Balqis, U., Tiuria, R., Hambal, M., dan Samadi, 2008. Kajian Titer Antibodi Pada Yolk Dari Ayam Yang Diimunisasi Dengan Antigen Ekskretori/Sekretori Stadium L3 Ascaridia galli. J. Agripet. 8(2):21-26.

Darmawi, Balqis, U., dan Hambal, M., 2009. Deposit Antibodi Anti-Ekskretori/ Sekretori Ascaridia galli di Dalam Yolk Ayam Petelur. Prosiding Seminar Nasional dan Pameran Hasil-Hasil Penelitian (Dalam Rangka Dies Natalis UNRAM ke 47), Mataram. 29-30 September. pp:304-312.

Darmawi, Balqis, U., Tiuria, R., Hambal, M., dan Samadi, 2010. Purifikasi Imunoglobulin Yolk Pada Ayam Yang Divaksin Terhadap Ekskretori/ Sekretori Terhadap Stadium L3 Ascaridia galli. J. Agripet. 10(2):9-15.

Davis, C., and Reeves, R., 2002. High Value Opportunities From the Chicken Egg. A Report for the Rural Industries Research and Development Corporation. RIRDC Pup.

Ding, L., and Candido, E. P. M., 2000. Association of several small heat-shock proteins with reproductive tissue in the nematode Caenorhabditis elegan. Biochem. J. 341:13-17.

Haak-Frendscho, M., 1994. Why IgY? Chicken Polyclonal Antibody, an Appealing Alternative. Promega Notes Magazine (46):11.

Harnett. W., MacDonald, M., Preece, G., Patterson, M., and Parkhouse, M. E., 1997. Production of monoclonal antibodies against excretory-Secretory products of adult male onchocerca gibsoni. J. of Parasitol. 83(2):316-319.

Hintz, 1998. juvenile female listomosoides sigmodontis produce an excretory-secretory antigen (Juv-p120) highly modified with dimethylaminoetha-nol. J. of Parasitol. 171:265-271.

Makoto, S. C., Robert, F., and Shuryo, N., 1998. Anti- E. coli immunoglobulin Y isolated from yolk egg of immunized chicken as a potensial food ingredient. J. of Food Sci. 53:1361-1365.

Mine, Y., and Kovacs-Nolan , J., 2002. Chicken egg yolk antibodies as therapeutics in enteric infectious disease: a review. J. Med. Food. 5:159-169.

Rhoads, M., L., Fetterer, R. H., and Urban, Jr. J. F., 2001. Release of Hyaluronidase During in vitro Development of Ascaris suum from the Third to Fourth Larval Stage. Parasitol. Res. 87(9): 693-697.

Roitt, I. M., and Delves, P. J., 2001. Roitt’s Esential Immunology. Tenth Edition, Blackwell Science Ltd. Osney Mead Oxford OX2 OEL.

Ruiz, A., 2004. Immunoprotection in Goats Against Haemonchus contortus After Immunization With Cysteine Protease Enriched Protein Fractions. Vet. Res. 35:565-572. http://www.edpsciences.org/articles/vetres/pdf/2004/05/v4019.pdf. [12 Agustus 2006]

Sander, J. E., and Schwartz, R. D., 1994. Evaluation of three water-suspensible formulations of fenbendazole against ascaridia galli infection in broiler chickens. Avi. Dis. 38:350-353.

Schou, T., Permin, A., Roupstorff, A., Sørensen, P., and Kj?r, 2003. Comparative Genetic Resistance to Ascaridia galli Infections of different Commercial Layer-lines. Brit. Poult. Sci. 44(2):182-185. http://orgprints.org/00001858 [21 Mei 06]

Schmidt, P., Wiedemann, V., Kühlmann, R., Wanke, R., Linckh, E., and Lösch, U., 1989. Production of antibodies to canine distemper virus in chicken eggs for Immunochemistry. J. of Vet. Med. B 36:661-668.

Soulsby, E. J. L., 1982. Helminth, Arthropods and Protozoa or Domesticated Animals. 7 th Ed. Lea and Febiger. Philadelphia.

Steel, R. G. D., and Torrie, J. H., 1999. Prinsip dan Prosedur Statistika: Suatu Pendekatan Biometrik. Edisi ke-2. Gramedia Pustaka Utama, Jakarta.

Taiwo, V. O., Alaka, O. O., Sadiq, N. A., and Adejinmi, J. O., 2002. Ascaridosis in captive reticulated python (python reticulatus). Afr. J. Biomed. Res. 5:93-95.

Tiuria, R., 1991. Hubungan Antara Dosis Infeksi, Biologi Ascaridia galli dan Produktivitas Ayam Petelur. Tesis, Program Pascasarjana. Program Studi Sains Veteriner. Institut Pertanian Bogor.

Tizard, I. R., 1996. Veterinary Immunology an Introduction. Fifth Edition, WB Sounders Company, a Division of Harcourt Brace and Company. The Curtis Center Independence Square West. Philadelphia, Pennsylvania.

Vervelde, 2003. Vaccination-induced Protection of Lambs Against the Para-sitic Nematode Haemonchus contortus Correlates With High IgG Antibody Responses to the LDNF Glycan Antigen. Glycobiol. 13(11):795-804.

Waller, P. J., 1997. Anthelmintic Resintance. Veterinary Parasitology. 72:391-412.

Zhang, W., Moreau, E., Peigné, F., Huang, W., and Chauvin, A., 2005. Comparison of Modulation of Sheep, Mouse and Buffalo Lymphocyte Responses by Fasciola hepatica and Fasciola gigantica Excretory-Secretory Products. http://parasitology.informatik.uni-wuerzburg.de/login/n/h/j_436_005-1306-x.html.html. [21 Februari 2006]




DOI: https://doi.org/10.17969/agripet.v11i2.370

Refbacks

  • There are currently no refbacks.


Copyright (c)




Creative Commons LicenseISSN: 1411-4623E-ISSN: 2460-4534
Copyright© 2000-2020 | ISSN: 1411-4623 | EISSN: 2460-4534 
Jurnal Agripet is licensed under a Creative Commons Attribution 4.0 International License.

 

Published by: 
Animal Husbandry DepartmentThe Faculty of Agriculture, Syiah Kuala University 
associated with Animal Scientist's Society of Indonesia (HILPI)
Jl. Tgk. Hasan Krueng Kalee No. 3, Kopelma Darussalam,
Banda Aceh, 23111, Indonesia.
Phone: +62-81383736633
Email: samadi177@unsyiah.ac.id & info.agripet@gmail.com


Online Submissions & Guidelines Editorial Policies | Contact Statistics Indexing | Citations