Deteksi Antibodi Serum Terhadap Virus Avian influenza pada Ayam Buras

Darmawi Darmawi, Zakiyah Heryawati Manaf, Darniati Darniati, Fakhrurrazi Fakhrurrazi, Mahdi Abrar, Erina Erina


Detection on Serum Antibodies of Native Chickens to Avian influenza Virus

ABSTRACT.  An important approach of controlling against Avian Influenza should be determined to detect the antibody titres of bird flu caused by Influenza virus H5N1 in Indonesia. The aim of the present study was to detect the antibodies to Avian Influenza in serum of native chickens. This study utilized 123 serum samples collected from the axilaris vein (left or right) of native chickens. Antibody titres were examined using Hemaglutination Inhibition (HI). The result showed that indication of natural infection by Avian Influenza (H5N1) in native chickens, as shown that out of 123 serum samples, 16 (13,01%) were tested positive by HI, while only 10 (8,13%) were tested protective to Avian influenza infection. Based on the results we obtained, a conclusion that natural infection by Avian influenza virus stimulated variety level of formation antibody titres in native chickens.


Avian influenza; antibody; native chickens

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Arsan Z., Febrianto, I., Collins, D., and Philippa, J., 2008. Evidence of Avian Influenza viruses of the H5 subtype in wild wandering whistling ducks (Dendrocigna arcuata) captured in Kalimantan for human consumption. Proceeding of AZWMC: 64 – 65. Bogor,19 – 21 August.

Basri C., Noor, G.M.S., Jatikusumah, A., dan Sunandar., 2008. Deteksi Sirkulasi virus Avian influenza H5N1 pada tempat penampugan ayam di Propinsi DKI Jakarta. Proceeding of AZWMC: 295 – 297. Bogor, 19 – 21 August.

Camenisch G., Tini, M., Chilov, D., Kvietikova, I., Srinivas, V., Caro, J., Spielmann, P., Wenger, R. H., and Gassmann, M., 1999. General Applicability of Chicken Egg Yolk Antibodies: the Performance of IgY Immunoglobulins Raised Against the Hypoxia-inducible Factor 1. J. FASEB. 13: 81-88.

Carlander D.. 2002. Avian IgY Antibody in vitro and in vivo. Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 1119, 53 pp. ACTA Universitatis Upsaliensis, Upsala.

Darmawi, Hambal, M., dan Fakhrurrazi., 2010. Purifikasi Imunoglobulin yolk anti-Avian influenza dari kuning telur ayam arab. Proceeding: National conference on chemical engineering and applications (ChESA), 538 - 547. Banda Aceh, 22 – 23 Desember.

Darmawi dan Hambal, M., 2011. Respon antibodi serum ayam Breakel silver terhadap vaksin Avian influenza. J. Kedokteran Hewan, 5(2): 63 – 66.

Hammond, E., 2007. Some Intellectual Property Issues Related to H5N1 Influenza Viruses, Research and Vaccines. The Sunshine Project. Third World Network. (20 Juli 2008)

Hoffmann, E., Lipatov, A.S., Webby, R.J., Govorkova, E.A., and Webster, R.G., 2005. Role of Specific Hemagglutinin Amino Acids in the Immunogenicity and Protection of H5N1 Influenza Virus Vaccines. Proceeding of the National Academy of Sciences of the United States of America (PNAS). 102 (36): 12915 – 12920. (26 Desember 2006)

Indriani, R., Indi, N.P.L., Darminto, D., dan Adjid, R.M.A., 2008. Survey Avian influenza pada pasar unggas hidup, titik kritis untuk pengambilan sampel. Proceeding of AZWMC: 261 -262. Bogor, 19 – 21 August.

Karaca, K., Swayne, D.E., Grosenbaugh, D., Bublot, M., Robles, A., Spackman, E., and Nordgren, R., 2005. Immunogenicity of Fowlpox Virus Expressing the Avian Influenza Virus H5 Gene (TROVAC AIV-H5) in Cats. Clin Diagn Lab Immunol. 12(11): 1340–1342. (26 Desember 2006).

Kurniawan, R.A., dan Dwiyanto, R., 2008. Deteksi antibody Avian influenza H5N1 pada kucing jalanan (Felis silvestris catus) di wilayah Semarang. Proceeding of AZWMC: 311 – 312. Bogor, 19 – 21 August.

OIE, 2000. OIE Manual of Standards for Diagnostic Test and Vaccines, 4th ed. Paris: Office International des Epizooties. p: 216.

Pamungkas, J., Iskandriati, D., Putra, M.A., and Setiawan, D., 2008. Antibodies to H5 Subtype of Avian influenza viruses in Macaca fascicularis in Indonesia. Proceeding of AZWMC: 149 – 150. Bogor, 19 – 21 August.

Purnamawati, A., dan Sudarnika, E., 2008. Kajian hasil vaksinasi Avian influenza pada ayam buras rakyat di Kabupaten Tasikmalaya. Proceeding of AZWMC: 281 – 283. Bogor, 19 – 21 August.

Rimmelzwaan, G.F., Kuiken, T., G. van Amerongen, Bestebroer, T.M., Fouchier, R.A.M., and Osterhaus, A.D.M.E., 2001. Pathogenesis of Influenza A (H5N1) Virus Infection in a Primate Model . Journal of Virology, p. 6687 - 6691, 75(14) (26 Desember 2006)

Rimmelzwaan, G.F., van Riel, D., Baars, M., Bestebroer, T.M., van Amerongen, G., Fouchier, R.A.M., Osterhaus, A.D.M.E., and Kuiken, T., 2006. Influenza A Virus (H5N1) Infection in Cats Causes Systemic Disease with Potential Novel Routes of Virus Spread within and between Hosts. American J. of Path.. 168:176-183. (26 Desember 2006).

Tarigan, S., Darminto, Loth, L., Indriani, R., dan Indi, N.L.P., 2008. Infeksi virus flu burung pada kucing di Jawa Barat. Proceeding of AZWMC: 308 – 310. Bogor, 19 – 21 August.

Wijanarko, Hadi, S., dan Kalianda, J.S., 2008. Surveilans program pembebasan Avian influenza (AI) di Kalimantan. Proceeding of AZWMC: 270. Bogor, 19 – 21 August.



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