Tingkat Kematangan Inti Oosit Sapi Setelah 24 Jam Presevasi Ovarium

Rini Widyastuti, Siti Darodjah Rasad

Abstract


(Nuclear maturity of bovine oocyte after 24 hours ovary preservation)

ABSTRACT. The objective of the research was to investigate  their meiotic competence or nuclear maturity of bovine oocytes maturity in vitro after 24 hour preservation on 5°C. Oocytes  were collected by slicing the ovaries in modified phosphate buffer saline (m-PBS). Selected cumulus-oocyte complexes (COCs) homogenous ooplasm  were cultured in maturity medium at 38°C in humidified atmosphere of 5% CO2 incubator. After 24 hours, oocytes stained for nuclear maturity’s evaluation. The proportion of oocytes at metaphase II (MII) was significantly difference (P< 0.05) on oocytes that 24 hours preservated   (44.21 ± 3.04%) vs oocytes from fresh ovary (73.97 ± 9.32%) (P<0.05). These results indicated that 24 hour’s preservation  bovine’s ovary on 50C  cause  decreases of nuclear oocyte maturity.


Keywords


Ovary; cumulus-oocyte complexes; preservation; maturity

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DOI: https://doi.org/10.17969/agripet.v15i2.2417

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