18. The Effect of Sipatah-Patah (Cissus quadrangularis Salisb) Extract on Mandible Density of Ovariectomized Rats (Rattus norvegicus)

Indah Maghfirah, Muhammad Jalaluddin, Dian Masyitha, Mustafa Sabri, Fadli A Gani, Herrialfian Herrialfian


The aim of this research was to identify the effect of sipatah-patah (Cissus quadrangularis Salisb) leaf extract on mandible density of ovariectomized rats (Rattus norvegicus). The samples were 12 female rats which were divided into 4 treatment groups with 3 replications. K0 consisted of ovariectomized rats without Cissus quadrangularis Salisb exstract, wheares K1, K2 and K3 consisted of ovariectomized rats  and were given Sipatah-patah extract with 500mg/kg body weight, 700/mg/kg body weight and 900mg/kg body weight for 30 days. On 31st day, rats were euthanized with cloroform and mandible bones were collected for histological preparation. Parameters observed were bone structures (osteosit, osteoblast, osteoclast and trabekula). The results showed that in K0 group, bone density was decreased and became thinner, osteoclast cells were found on trabekula, and low density of active and passive osteoblast were also detected. The treatment group (K1 and K2) showed an improvement stucture of trabekula,where the amount of osteoclast were lower than K0, as well as the amount of active osteoblast, although a lot of passive osteoblast were still found. The treatment group (K3) showed an improvement as the most dense trabekula structure with large amount of active osteoblast cell density without the existance of osteoclast compared to other grups. In this research, it would be concluded that the adding sipatah-patah extract with a dose of 900 mg/kb body weight showed a higher number of active osteoblasts and trabekula density compared to the control groups, K1, and K2 on ovariectomized mandible bone rats


Sipatah-patah (Cissus quadrangularis Salisb); osteoporosis; ovariectomy; Mandible Bones; osteosit; osteoblast; osteoclast and trabekula

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Almatsier, 2004. Prinsip Dasar Ilmu Gizi Jakarta, Gramedia Pustaka Utama.

Arjmandi BH, Alekel L, Hollis BW, Amin D, Stacewiez-Sapuntzakis M, Guo P and Kukreja SC. 1996. Dietary soybean protein prevents bone loss in an ovariectomized rat model of osteoporosis. J. Nutr. 126:161-167.

Arjmandi, BH. 1996. Dietary soybean protein prevents bone loss in an ovariectomized rat model of osteoporosis. J. Nutr. 126(1):161-167.

Banks, WJ. 1993. Applied Veterinary Histology. 3rd Ed : Mosby Year Book. Toronto.

Corwin dan Elizabet. 2001. Buku Saku Patofisiologi. EGC, Jakarta.

Dewell, A., B.H. Clarie, and B. Bonnie. 2002. The effect of soy-derived phytoestrogens on serum lipids and lipoprotein in moderately hypercholesterolemic postmenopausal women. J. Clin. Endocrin. Metab. 87(1):118-121.

Eklou-Kalonji, E., E. Zerath, C. Colin, C. Lacroix, X. Holy, I. Denis and Pointtilart. 1999. Calcium regulating hormones, bone mineral content, breaking load and trabecular remodeling are altred in growing pigs fed calsium–deficient diets. J. Nutr. 129: 188-193.

Eriksen, EF., Axelrod DW, and Melsen F. 1994. Bone Histomorphometry. Raven press, New York.

Jainu, J.M. dan Devi, C.S.S. 2006. Gastroprotective effect of Cissus quadrangularis extract in rats with experimentally induced ulcer. Indian J. Med. Res. 123:799-806.

Jubb, K.V.F., P. C. Kennedy, and N. Palmer. 1991. Pathology of Domestic Animal. 3rd ed: Academic Press, Inc. San Diego.

Kalu, DN, Elena S, Chung-Ching L, Fabrizio F, Arjmandi BH, Mohammed, A and Salih. 1993. Ovariectomy-induced bone loss and the hematopoetic system. Bone and Mineral. 23:145-161.

Kiernan, J.A. 1990. Histological and Histochemical Method: Theory and Practice. 2nd ed. Pergamon Press, New York.

Kierszenbaum, A.L. 2002. Histology and Cell Biology: An Introduction to Pathology Inc. An Affiliate of Elsevier. . Mosby. St. Louis.

Krane, S.M. 1974. Metabolic Bone Disease: Principle of Internal Medicine. Edisi 7. JMC Press Inc. Quezon City, Filipina.

Mahaputra, L. 2002. Reseptor estrogen pada mencit menopause dan masih bersiklus reproduksi. J. Biosains. 4:65-68.

Masyitha, D. 2003. Struktur mikroskopik tulang ischium pada tikus ovarektomi dan pemberian pakan rasio fosfat/kalsium tinggi. Media Kedokteran Hewan. 19(1): 21-24.

Masyitha, D. 2006. Struktur mikroskopik tulang mandibula pada tikus ovariektomi dan pemberian pakan rasio fosfat/kalsium tinggi. Media Kedokteran Hewan. 22(2):112–7.

Nieves. 2005. Osteoporosis: the role of micronutrients. J. Am. Clin. Nutr. 81:1232S-1239S.

Oursler, M.J. 2003. Direct and indirect effects of estrogen on osteoclast. J. Musculoskel Neuron Interact. 3(4):363-6.

Rachman, I.A. 2006, Osteoporosis primer (Post menapouse osteoporosis). In:Osteoporosis. (Editor: Suherman SK, Tobing S Dohar AL). Edisi I. Perhimpunan osteoporosisi Indonesia. Indomedika:1-6.

Sabri, M. 2011. Aktivitas Ekstrak Etanol Batang Sipatah-patah (Cissus quadrangula Salisb) Sebagai Antiosteoporosis Pada Tikus (Rattus norvegicus). Disertasi. Sekolah Pasca Sarjana. Institut

Pertanian Bogor. Bogor

Sabri, M. 2013. Administration’s effects of ethanol extract of Cissus quadrangularis Salisb on growth of lumbal bone ovariectomizwd rats. Jurnal Natural. 12(2):48-49.

Shirwaikar, A.N. S. Khan, and S. Malini. 2003. Antiosteoporotic effect of ethanol extract of Cissus quadrangularis Linn. on ovariectomized rat. J. Ethnopharmacol. 89:245-250.

Suleiman, S., M. Nelson, F. Li, M. Thomas, B and C. Moniz. 1997. Effect of calcium intake and physical activity level on bone mass and turnover in healthy, white, postmenopausal women. J. Am. Clin. Nutr. 66:937 -43.

Sumiyati dan Sunaryo. 1998. Penanggulangan Osteoporosis Imbas Panhisterovariektomi dan Diet Rasio Kalsium/Fosfat Tinggi dengan Rebon pada Tikus. Penelitian DPP Mandiri UGM: 13-22.

Thuong, P.T. W. Jin, J. Lee, R. Seong, Y.M. Lee, Y. Seong, K. Song, K. Bae. 2005. Inhibitory effect on TNF-alpha-induced IL-8 production in the HT29 cell of constituents from the leaf and stem of Weigela subsessilis, Arch. Pharm. Res. 28:1135–1141

Turner, J.V, Agatonovic-Kustrin S, Glass BD. 2007. Moleculer aspects of phytoestrogen selective binding at estrogen reseptor. J. Pharm. Sci. 96(8):1879-1885.

Winarno B. 1998. Densitas mineral tulang wanita menyusui. Tesis. Program Pendidikan Dokter Spesialis. Fakultas Kedokteran Universitas Indonesia, Jakarta.

Zhang, Y. Ping, W. Leung, P.C. Wu. C.F. Yao, X.S and Wong, M.S. 2006. Effects of fructus Ligustri lucidi extract on bone turnover and calcium balance in ovariectomized rats. Biol. Pharm. Bull. 29(2):291-296.

DOI: https://doi.org/10.21157/j.med.vet..v12i2.4307

DOI (PDF): https://doi.org/10.21157/j.med.vet..v12i2.4307.g9102


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