20. The Isolation of Salmonella sp. on quail eggs (cortunxi-cortunix japonica) that failed to hatch in Garot, Darul Imarah Subdistrric, Aceh Besar

Latifa Suryandari, Erina Erina, Darniati Darniati, Safika Safika, Nuzul Asmilia, M. Nur Salim

Abstract


The study aimed to isolate the Salmonella sp on quail eggs that failed to hatch in Garot village, Darul Imarah Subdistrict, Aceh Besar. A total of thirty quail eggs that failed to hatch were examined in microbiology laboratory of Veterinary Medicine Faculty, Syiah Kuala University. Modification of Carter method was used in this study to isolated samples. The eggs were opened for taking the embryo and then it was swabbed with sterile swabs.  The swab was put into Selenite Cystine Broth (SCB) and incubated at 37o C for 24 hours. The breed was then transferred onto Salmonella Shigella Agar (SSA). The growing Bacteria were observed for their morphology and Gram staining to make sure the bacteria belonged to the negative Gram. The result showed that 10 of the 30 of quail eggs that failed to hatch were positively infected with Salmonella sp. Based on this study, it can be concluded that the salmonella sp.can be isolated from quail eggs which were not hatched in the farm of Garot, Darul Imarah Subdistrict, Aceh Besar

Keywords


quail egg; Salmonella sp; SCB; SSA

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References


Amarantini, C., Widya, A., Haripurnomo, K., dan Langkah Sembiring. 2009. Seleksi bakteri salmonella typhi dari kultur darah penderita demam tifoid. Prosiding Seminar Nasional Penelitian, Pendidikan dan Penerapan MIPA . Fakultas MIPA, Universitas Negeri Yogyakarta, Yogyakarta.

Blankeship, L. C., Bailey, J. C., Cox, N. A., Stern, N. J., Brewer, R., and Wiliams, O. 1993. Two step mucosal competitive exclusion flora treatment to diminish salmonellae in commercial broiler chikens. Poult Sci 72(9): 1667-1672.

Bridson, E.Y. 2006. The Oxoid Manual. Ed 9th. Oxoid Limeted, England

Carter, G.R. 1976. Essential Of Veterinary Bacteriology and Mycology. Michigan State University East Lansing, Michigan.

Cason, J. A., Cox, N. A., and Bailey, J. S. 1994. Transmission of salmonella typhimurium during hatching of broiler chicks. Avian Dis 38(3):583-588.

Cox, N. A., Bailey, J. S. Muldin, J. M., Blankenship, L. C., and Wilson, J. L. 1991. Extent salmonella contamination in the breeder hatcheries. Poult Sci 70(2): 416-418.

Darmono dan Darminto. 2001. Permasalahan Penyakit Sebagai Kendala Usaha Peternakan Itik (Important Diseases in Duck Farming). Balai penelitian Veteriner.

Elvioleta., I. 2015. Isolasi Salmonella sp. Pada Burung Puyuh (Coturnix-coturnix japonica) Di Kecamatan Darul Imara, Aceh Besar. Skripsi. Fakultas Kedokteran Hewan, Universitas Syiah Kuala. Banda Aceh.

Istiana. 1998. Keberadaan Salmonella sp pada telur yang gagal menetas dan anak itik pasca tetas di pusat penetasan itik alabio di kabupaten Hulu Sungai Utara Kalimantan Selatan. Media Veteriner. 5(2): 27-31.

Jayasamudera, D. J dan B. Cahyono. 2005. Pembibitan Itik. Penebar Swadaya, Jakarta.

Katayama, E., T. C. Danato., A. D. P. Silva., R. P. Mazola., E. A. Garcia., A. S. Okamoto., and R. L. Andreatti. 2013. Salmonella Entritidis in the eggs of japonica quails (Cortunix cortunix japonica). Avic press, Brazil 15(1).

Kusuma, S. A. F. 2009. Uji Biokimia Bakteri. Karya Ilmiah. Fakultas Farmasi. Universitas Padjadjaran, Bandung.

Listiyowati, E dan Roospitasari, K. 2007. Burung Puyuh Tata Laksana Budidaya Secara Komersial. Penebar Swadaya, Jakarta.

Nugroho, dan I. G. T. Manyun,. 1981. Beternak Burung Puyuh. Eka Offest, Semarang.

Pelczar. M. J dan E. S. C. Chan. 2005. Dasar-Dasar Mikrobilogi 2. UI Press, Jakarta.

Rao, S. V. R., D. Nagalakshmi and V. R. Reddy. 2002. Feeding to minimise heat stress. Poultry International. 41(7). 30-33.

Ray, B. 2001. Fundamental Food Microbiology. Ed Ke-2. Boca Raton Florida: CRC Press.

Retno, T. D. N. 2009. Perbandingan Metode SNI Dengan Metode Salmonella Latex Test Untuk Memantau Pencemaran Salmonella spp. Pada Kotak Pengangkutan Day Old Chick (DOC). Tesis. Sekolah Pasca Sarjana. Institut Pertanian Bogor, Bogor.

Rochan, R.c., W. M. Cardose., R. S. C. Teixeira., A. H. Albuquerqu., R., V, Horn., C. M. Cavalcanti., E. S. Lopes., and V. J. R. Gomes Filho. 2013. Salmonella gallinarum virulence in experimentally-infected japanese quails ( Coturnix Japonica). Avic press, Brazil 15(1). 281-286.

Shivaprasad, G.H. 1997. Pullorum Diesease and Fowl Typhoid. Disease Of Poultry 10 ens. Lowa state University Press, USA.

Srianta dan Elisa. R. 2003. Deteksi salmonella pada nasi goreng yang disediakan oleh restoran kereta api kelas ekonomi. Teknologi Pangan. Universitas Katolik Widya Mandala Surabaya, Surabaya. Teknol dan Industri Pangan. 14(3): 253-257.

Tabbu, C. R. 2000. Penyakit Ayam dan Penanggulangannya. Penerbit Kanisius, Yogyakarta.

Thrusfield, M. 2005. Veterinery Epidemiology. Ed 3rd. Blackwell Publisher Company, London

USDA (United States Departement of Agriculture). 2016. USDA National Nutrient Database for Satandard Reference. https://ndb.nal.usda.gov /ndb/search. 30 Mei 2016

Yuwanta, T. 1993. Perencanaan dan Tata Laksana Pembibitan Unggas. Inseminasi Buatan pada Unggas. Fakultas Peternakan, UGM, Yogyakarta.

Yuwanta, T. 2004. Dasar Ternak Unggas. Kaninus, Yogyakarta.




DOI: https://doi.org/10.21157/j.med.vet..v12i2.4118

DOI (PDF): https://doi.org/10.21157/j.med.vet..v12i2.4118.g9104

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