2. The Effect Of Sipatah-patah (Cissus quadrangularis Salisb) Extract On The Femur Bone Density of White Rat (Rattus norvegicus) with Model Ovariectomy

Mustafa Sabri, Debby Novita ayumi, Muhammad Jalaluddin, Hamny Hamny, Cut Dahlia Iskandar, Herrialfian Herrialfian

Abstract


This research aimed to determine the effect of giving Sipatah-patah extract toward the histopathological and femur bone growth of white rat that was ovariectomized. The experimental animals that were used were 12 white rats divided into 4 treatment group with 3 repetitions. K0 was the ovariectomized rat without giving Sipatah-patah extract (ESP); K1, K2 and K3 were ovariectomized rats which were given Sipatah-patah extract with multilevel doses of 500 mg/kg BW, 700 mg/kg BW and 900 mg/kg BW for 30 days. On the 31st day, rats were euthanized using chloroform and os femur that was taken to being made into histological preparation.  There was the decrease in the bones density of the K0 group which is characterized by thinning of trabecular structure, there were lots of osteoclast cells on the edge of the trabecular and lower density of active osteoblasts and passive osteoblasts. The rat of group K1 and K2 showed an improvement on the trabecular structure and lower osteoclast than group K0. The rat of group K3 had a visible improvement of the most congested trabecular structures, cohesive with the most density cell of active osteoblasts than the other groups. The result of this research concluded that the giving of Sipatah-patah extract doses 900 mg/kg BW showed a higher density of trabecular and active osteoblasts than the control group, K1 and K2 on the white rat bones that were ovariectomized.

Keywords


white rat; ovariectomy; Sipatah-patah extract; os femur; trabecular; osteoclast and osteoblast cells.

Full Text:

PDF

References


Anggraini, W. 2008. Fitoestrogen sebagai alternatif alami terapi sulih hormon untuk pengobatan osteoporosis primer pada wanita pascamenopouse. M.I. Kedokteran Gigi. 23(1): 25-31.

Baziad, A. 2003. Menopause dan Andropause. Edisi ke-1. Yayasan Bina Pustaka, Jakarta.

Bell, N. H. 2003. Rank ligand and the regulation of skletal remodeling. J. Clin. Invest. 111:1120-1122.

Ceriana, R., I. Djuwita, dan T. Wresdiyati. 2014. Ekstrak batang Sipatah-patah meningkatkan proliferasi dan diferensiasi sel punca mesenkimal sumsum tulang. Jurnal Veteriner 15(4): 436-445.

Cunningham, J. G. 1992. Texbook of Veterinary Physiology. W. B. Saunders Company, Philadelphia.

Djuwita, I., A.P. Irma, W. Adi, dan M. Sabri. 2012. Proliferasi dan diferensiasi sel tulang tikus dalam medium kultur in vitro yang mengandung ekstrak batang cissus quadrangula salisb (Sipatah-patah). Jurnal Kedokteran Hewan. 6(2):75-80.

Fefridayanti, D. 2012. Pemberian Ekstrak Tanaman Sipatah-patah (Cissus quadrangularis Salisb Terhadap Gambaran Histologi Dan Densitas Tulang Lumbal Tikus Putih (Rattus norvegicus) Yang Diovariektomi. Skripsi. Fakultas Kedokteran Hewan, Universitas Syiah Kuala, Banda Aceh.

Hartiningsih., D.A, dan I. Widiyono. 2010. Pengaruh Panhisterektomi dan Konsumsi Suplemen 1,25-Dihidroksivitamin D3 Selama 1,5 Bulan Terhadap Retensi Kalsium Pada Tikus Wistar. Laporan Penelitian. Lembaga Penelitian Universitas Gadjah Mada, Yogyakarta.

Hartiningsih, D.A. dan D. Aji. 2012. Respons metafisis tulang femur distalis tikus ovariektomi yang mengkonsumsi kalsitriol. Jurnal Kedokteran Hewan 6(2): 92-98.

Jainu, M., K. Vijaimohan, and D.C.S. Shyamala. 2006. Gastroprotective effect of Cissus quadrangularis extract in rats with experimentally induced ulcer. J. Ethnopharmacol. 123(6): 799-806.

Kiernan, J. A. 1990. Histological and Histochemical Method: Theory and Practice. 2nd ed. Pergamon Press, New York.

Kusec, V., A.S. Virdi, R. Prince, and J.T. Triffitt. 1998. Localization of estrogen receptor-alpha in human and rabbit skeletal tissues. JCEM. 83(7): 2421-2428.

Leeson, R.C., T.S. Leeson, dan A.A. Paparo. 1996. Buku Ajar Histologi Edisi VII. Terjemahan Tambayong et al, EGC, Jakarta.

Mahaputra, L. 2002. Reseptor estrogen pada mencit menopause dan masih bersiklus reproduksi. J.B.P. 2(4): 65-68.

Martin, T.J. 1993. Hormones in the coupling of bone resorption and formation. Osteoporosis Int. 3(1): 121-125.

Masyitha, D. 2006. Struktur mikroskopik tulang mandibula pada tikus ovarektomi dan pemberian pakan rasio fosfat/kalsium tinggi. Media Kedokteran Hewan 22(2): 112-117.

Nelson, N.L, and S.E. Bulun. 2001. Estrogen production and action. J. Am. Acad Dermatol. 45(3): 116-124.

Nilsson, S.K., M.S. Dooner, H.U. Weier, B. Frenkel, J.B. Lian, G.S. Stein, and P.J. Quesenberry. 1999. Cells capable of bone production engraft from whole bone marrow transplants in nonablated mice. J. Exp. Med. 189(4): 729-734.

Potu, B.K., M.R.B. Kumar, M.S. Rao, G.K. Nampurath, M.R. Chamallamudi, S.R. Nayak, and M.S. Muttigi. 2009. Petroleum ether extract of Cissus quadrangularis (Linn.) enhances bone marrow mesenchymal stem cell proliferation and facilitates osteoblastogenesis. Clinical Science. 64(10): 993-998.

Prasetya, R.O. 2012. Studi Pemberian Tepung Tulang Ikan Tuna Madidihang (Thunnus albacares) Pada Hewan Model Ovariektomi Tikus (Rattus norvegicus) Terhadap Gambaran Histopatologi Dan Ekspresi Il – 1 Β Dari Caput Humeri. Skripsi. Program Studi Kedokteran Hewan, Universitas Brawijaya, Malang.

Pratomo, F.A. 2012. Efek Pemberian Tepung Tulang Ikan Tuna Madidihang (Thunnus albacares) Pada Tikus Putih (Rattus norvegicus) Model Ovariektomi Berdasarkan Histopatologis Tulang Femur Dan Ekspresi Tnf-Α. Skripsi. Program Studi Pendidikan Dokter Hewan, Universitas Brawijaya, Malang.

Rao, M.S., B. Kumar, V.B.N. Swamy, and N.G. Kutty. 2007. Cissus quadrangularis plant extract enhances the development of cortical bone and trabecular in the fetal femur. Pharmacologyonline 3: 190-202.

Sabri, M., Nurhidayat, K. Sigit, B.P. Priosoeryanto, dan W. Manalu. 2009. Analysis of phytochemical and mineral content of Sipatah-patah plant (Cissus quadrangularis) from Aceh as osteoporosis premedication. Jurnal Rona Lingkungan 1(2): 109-117.

Sabri, M. 2011. Aktivitas Ekstrak Etanol Batang Sipatah-patah (Cissus quadrangularis Salisb) Sebagai Antiosteoporosis Pada Tikus (Rattus norvegicus). Disertasi. Program Studi Sains Veteriner, Sekolah Pascasarjana. IPB, Bogor.

Sabri, M. 2013. Administration’s effects of ethanol extract of cissus of lumbal bone in ovariectomized rats. ovariectomized rats. Jurnal Natural 13(2): 48-54.

Samuels, A., M.J. Perry, A.E. Goodship, W.D. Fraser, and Tobias. 2000. Is high-dose estrogen-induced osteogenesis in the mouse mediated by an estrogen receptor?. Bone. 27(1): 41-46.

Samuelson, D. A. 2007. Textbook of Veterinary Histology. Elsevier Health Sciences, London.

Shirwaikar, A., S. Khan, and S. Malini. 2003. Antiosteoporotic effect of ethanol extract of Cissus quadrangularis Linn. on ovariectomized rat. J. Ethnopharmacol. 89(2): 245-250.

Syed, F.A., M.J. Oursler, T.E. Hefferan, J.M. Peterson, B.L. Riggs, and S. Khosla. 2008. Effects of estrogen therapy on bone marrow adipocytes in postmenopausal osteoporotic women. Osteoporos Int. 19(9): 1323-1330.

Trihapsari, E. 2009. Faktor-faktor Yang Berhubungan Dengan Densitas Mineral Tulang Wanita ≥ 45 Tahun Di Departemen Pendidikan Nasional Jakarta Pusat Tahun 2009. Skripsi. Program Studi Kesehatan Masyarakat, Universitas Indonesia.

Weise, M., S. De-Levi, K.M. Barnes, R.I. Gafni, V. Abad, and J. Baron. 2001. Effects of estrogen on growth plate senescence and epiphyseal fusion. PNAS. 98(12): 6871-6876.

Wirakusumah, E.S. 2003. Tips Dan Solusi Gizi Untuk Tetap Sehat, Cantik Dan Bahagia Di Masa Menapouse Dengan Terapi Estrogen Alami. Penerbit PT Gramedia Pustaka Utama, Jakarta.

Woo, D.G., C.Y.Ko, T.W. Lee, H.S. Kim, and B.Y. Lee. 2005. long-term study for the effect of ovariectomy on rat bone - use of in-vivo micro-CT. Journal

World Academy of Science, Engineering and Technology. 921-924.

Yamaguchi, M. 2002. Isoflavone and bone metabolism : its cellular mechanism and preventive role in bone loss. Journal of Health Science 48(3): 209-222.




DOI: https://doi.org/10.21157/j.med.vet..v13i1.4108

DOI (PDF): https://doi.org/10.21157/j.med.vet..v13i1.4108.g9963

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 by author and J. Med. Vet.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Creative Commons License
J. Med. Vet. is licensed under a Creative Commons Attribution 4.0 International License.