3. Lactobacillus casei Fermented Milk as a Treatment for Diabetes in Mice (Mus musculus)

Pratiwi Purnama Sari, Nurliana Nurliana, M. Hasan, Arman Sayuti, Sugito Sugito, Amiruddin Amiruddin

Abstract


The study aimed to find out the effect of Lactobacillus casei fermented milk as diabetic drug (Therapeutic). Mice were 3-4 month old, male and body weight ranged 20-30 g. This Research used Completely Randomized Design with factorial pattern, consisted of dose and  duration of  feeding. Treatments were divided into four treatment, P1 (no treatment), P2 (mice were induced with alloxan), P3 (mice were induced with alloxan and fed with fermented milk L. casei with 0.5 mL/mice) and P4 (mice were induced with alloxan and fed with Lactobacillus casei fermented milk 1 mL/mice). Provision of L. casei fermented milk provided at libitium and L. casei fermented milk given for 7 and 14 days. Blood  were collected from Vena lateralis and dropped on the strip test Easy Touch GGHb. The results showed that not only dose administration of L. casei fermented milk had significantly decrease (P<0.01) of glucose levels in mice, but also duration of treatment. It can be concluded that the administration of fermented milk L. casei can be used as a complement therapy for diabetic mice

Keywords


Fermented milk; antidiabetic; Lactobacillus casei; onset

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References


Adewani, N. 2008. Pengaruh Pemberian Rebusan Kulit Kayu Duwet Terhadap Persentase Penurunan Kadar Glukosa Darah Pada Tikus Putih. Skripsi. Universitas Muhammadiyah Yogyakarta. Yogyakarta.

Balflour, J.A. and D. McTavish. 1993. Acarbose. An Update of its Pharmacology and Therapeutic Use in Diabetes Mellitus. Drugs. 46:1025-1054.

Cahyanti, A.N. 2011. Viabilitas probiotik Lactobacillus casei pada yougurt susu kambing selama penyimpanan beku. Jurnal Teknologi Pertanian. 12(3):176-180.

Chen, P., Q. Zhang, H. Dang, X. Liu, F. Tian, J. Zhao, Y. Chen, H. Zhang, and W. Chan. 2014. Screening for potential new probiotic based on probiotic properties and α-glucosidase inhibitory activity. Food Control. 35:65-72.

Erwin, Etriwati, dan Rusli. 2012. Mencit (Mus musculus) galur BALB-C yang diinduksi streptozotosin berulang sebagai hewan

model diabetes melitus. Jurnal Kedokteran Hewan. 6:48-50.

FAO. 2001. Evaluation of health and nutritional properties of powder milk and live lactic acid bacteria. Food and Agriculture Organization of the United Nations and World Health Organization Report. http://www.fao.org/es/ESN/ Probio/probio.htm (20 Desember 2015).

Gesperz, V. 1989. Metode Perancangan Percobaan. Armico, Bandung.

Icks, A., B. Haastert, C. Trautner, G. Giani, G. Glaeske, and F. Hoffman. 2009. Incidence of lower-limb amputations in the diabetic compared to the non-diabeticpopulation. Findings from Nationwide Insurance Data. Germany 2005-2007. Experimental and Clinical Endocrinology & Diabetes. 117:4-500.

Jain, S. and S. Saraf. 2010. Type 2 diabetes mellitus-its global prevalence and therapeutic strategies. Diabetes and Metabolic Syndrome: Clinical Research and Reviews. 4(1):48-56.

Kusumaningtyas, I.D., S. Fajariyah, dan E.T. Utami. 2014. Pengaruh seduhan kayu manis (Cinnamomum burmanii) terhadap struktur pankreas mencit (Mus musculus) strain Balb-C diabetic. Jurnal Ilmu Dasar. 15(2):69-73

Lin, Y. and Z. Sun. 2010. Current views on type 2 diabetes. Journal of Endocrinol. 204(1):1-11.

Lye, H.S., C.Y. Kuan, J.A. Ewe, W.Y. Fung, and M.T. Liong. 2009. The improvement of hypertension by probiotics: effects on cholesterol, diabetes, renin, and phytoestrogens. International Journal of Molecular Sciences. 10(9):3755-3775.

Malik, M.I., E. Nasrul, dan Asterina. Hubungan hiperglikemia dengan prothrombin time pada mencit (Mus musculus) yang diinduksi aloksan. 2015. Jurnal Kesehatan Andalas. 4(1):182-188.

McCann, T., T. Egan, and G,H. Weber. 1996. Assay procedures for commercial probiotic cultures. J. Food Protection. 59:41–45.

Nighswonger, B.D., Brashears, M.M, and Gilliland, S.E. 1996. Viability of Lactobacillus acidophilus and Lactobacillus casei in fermented milk products during refrigerated storage. J. Dairy Sci. 79:212–219.

Rhom, H., F. Lechner, and M. Lehner. 1990. Microflora of Austrian natural-set yoghurt. J. Food Protection. 53:478–480.

Sangwan, S., and R. Singh. 2014. Therapeutic effects of probiotic fermented milk (LGG and L. casei NCDC 19) on progression of type 2 diabetes. Journal of Innovative Biology. 1 (2):78-83.

Shah, N.P. 2001. Functional foods from probiotics and prebiotic. Food Technology. 55(11):46-53.

Sunaryo, H., Siska, Dwitiyanti, dan R.A. Rizky. 2014. Kombinasi ekstrak etanol rimpang Zingiber officinale Roscoe dengan Zn sebagai hipolipidemia pada mencit diabetikdie tinggi kolesterol. Media Farmasi. 11(1):62-72.

Szkudelski, T. 2001. The mechanism of alloxan and sreptozotocin action in cells of the rat pancreas. Physiol. Res. 50:536-546.

WHO. 2001. Evaluation of health and nutritional properties of powder milk and live lactic acid bacteria. Food and Agriculture Organization of the United Nations and World Health Organization Report. http://www.fao.org/es/ESN/ Probio/probio.htm (20 Desember 2015).

WHO. 2009: Diabetes. http://www/who.int/. (24 Januari 2016).

Yamano, T., M. Tanida, A. Niijima, K. Maeda, N. Okumura, Y. Fukushima, and K. Nagai. 2006. Effects of the probiotic strain Lactobacillus johnsonii strain La1 on autonomic nerves and blood glucose in rats. Elsever. 79(20):1963-1967.

Yun, S. I., H.O. Park, and J.H. Kang. 2009. Effect of Lactobacillus gasseri BNR17 on blood glucose levels and body weight in a mouse model of type 2 diabetes. Journal of Applied Microbiology. 107(5):1681-168




DOI: https://doi.org/10.21157/j.med.vet..v11i1.4088

DOI (PDF): https://doi.org/10.21157/j.med.vet..v11i1.4088.g6890

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J. Med. Vet. is licensed under a Creative Commons Attribution 4.0 International License.