5. Isolation and Identification of Salmonella sp in spleen of male layer chicken in Sibreh farms, Aceh Besar

Erina Erina, Mahdi Abrar, Balqis Alrasyid Suyoto, Maryulia Dewi, Darmawi Darmawi, Darniati Darniati, Muttaqien Bakri

Abstract


This study aimed to isolate and identify the bacteria Salmonella sp in spleen of male layer chicken that died in farms Sibreh, Aceh Besar. A sample of the research is the spleen of male layer chickens. Isolation of Salmonella sp performed by inoculating spleen that were pierced using a swab on the media Selenite Cystine Broth (SCB) then planted on selective media Salmonella Shigella Agar (SSA) and colony morphology was observed, continued with observation of Gram stain under microscopic. Furthermore, colonies of suspected Salmonella sp planted in the media MR-VP (Oxoid), Indol (Difco), Simmons's Citrate Agar (Oxoid), Triple Sugar Iron Agar (TSIA), Sulfite Indol Motiliti (SIM), and media sugars (sucrose, manitol, glucose, lactose , maltose). The results of this study were analyzed descriptively. The research shows a results from a total of 33 samples, we found that 15 samples are positive that can be isolated and identified as Salmonella sp. Therefore it can be concluded that Salmonella sp is possible the death cause of male layer chicken in farms Sibreh, Aceh Besar

Keywords


male layer chiken; spleen; salmonella sp; farm

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References


Aktar, N. Rabeya, B, and M. Ilias. 2016. Isolation and Identification of Salmonella sp. from different food.Int. J. Biosci. 8(2) : 16-24.

Budiarso, T.Y. dan M.J.X. Belo. 2009. Deteksi cemaran Salmonella sp pada daging ayam yang di jual di pasar tradisional di wilayah Kota Yogyakarta. Prosiding Seminar Nasional Penelitian, Yogyakarta.

Carter, G.R. 1987. Essentials of Veterinary Bakteriology and Micology. 3rd ed. Lea and Febriger, Philadelphia.

Dundu, Bertus. 2000. Penuntun Praktikum Mikrobiologi. Jurusan Hama dan Penyakit Fakultas Pertanian Universitas Sam Ratulangi, Manado.

Firnanda, R., Sugito, Fakhrurrazi, D. Vianika, dan S. Ambarwati. 2013. Isolasi Aeromonas hydrophila Pada Sisik Ikan Nila (Oreochromis niloticus) Yang diberi tepung daun jaloh (Salix tetrasperma Roxb). JurnalMedika Veterinaria.Fakultas Kedokteran Hewan Universitas SyiahKuala, Banda Aceh.

Gibson, J. M. 1996. Mikrobiologi danPatologi Modern untuk Perawat.(Diterjemahkan I.K.G. Somaprasada). Penerbit Buku Kedokteran EGC, Jakarta.

Hadioetomo, R.S. 1985. Mikobiologi Dasar Dalam Praktek. Penerbit Gramedia, Jakarta.

Hidayat, O. F. A. Febria, dan N. Nasir. 2014. Isolasi dan karakterisasi bakteri pada pasir sarang dan cangkang telur penyu lekang (Lepidochelys olivaceaeL) yang menetas dan gagal menetas.J. Bio. UA. 3(2) : 154-161.

Kusuma, F.A.S. 2009. Uji Biokimia Bakteri. Karya ilmiah. Fakultas Farmasi Universitas Padjajaran, Bandung.

Listiyowati, E. dan K. Roospitasari. 2009. Beternak Puyuh Secara Komersial. Penebar Swadaya, Jakarta.

Nesheim, M.C. E.A. Richard, and E.C. Leslie. 1979. Poultry Production. 12th Ed. Lea and Febiger. Philadelphia.

OIE. 2008. Fowl typhoid and pullorum disease. OIE Terrestrial Manual, Paris, France.

Poppe, C. 1996. Salmonellosis In Poultry and People. Edisi Mei. In Word Poultry pp.

Portillo, D.F.G. 2000. Moleculer and Celluler Biology of Salmonella Pathogenesis microbial disease Mechanisms of pathogenesis and Toxin Synthesis. Techonomic Publishing Company,Inc. Cancaster, Pennsylvania, USA.

Quinn, P. J. B. J. Markey, M.E. Carter, W.J. Donnelly, and F.C. Leonard. 2002.Veterinary Microbiology and Microbial Disease. Garsinton Road,Oxford, United Kingdom: Blackwell Publishing Company.

Sarno. Dewi, H. 2007. Sistem Pengadaan Pakan Ayam Petelur di Perusahaan“Populer Farm” Desa Kuncen Kecamatan Mikjen Kabupaten Semarang.Skripsi. Fakultas Pertanian Universitas Wahid Hasyim, Semarang.

Shivaprasad, H.L. 2003. Pullorum disease and fowl typoid In Diseases ofPoultry. Section II Bacterial Diseases. 11thEdition. Iowa State University Press Ames, Iowa, USA.

Sudarmono, A.S., 2003. Pedoman Pemeliharan Ayam Ras Petelur. Penerbit Kaninus, Yogyakarta.

Suryani, Y. A. O. Bernadeta, dan U. Siti. 2010. Isolasi dan karakterisasi bakteri asam laktat dari limbah kotoran ayam sebagai agnesi probiotik dan enzim kolesterol reduktase. Prosiding Seminar Nasional Biologi. Universitas Negri Yogyakarta. Yogyakarta :138-147.

Tetty. 2002. Puyuh Si Mungil Penuh Potensi. Agro Media Pustaka, Jakarta.

Ulfah, M., Dwi, J.S. dan Sri, S. 2013. Meningkatkan Produksi Ayam Petelur.Edisi Revisi. Penerbit Niaga Swadaya, Depok.

Wasiudin, A. A. 2011. Strategi Pengembangan Usaha Ayam Petelur Jantan Pada UD Mangestoni Putri Poultry Shop di Desa Gadingsari Kecamatan Sanden Kabupaten Bantul. Skripsi. Institut Pertanian Bogor, Bogor.

Wuryadi. 2013. Beternak Puyuh. Penerbit Agromedia Pustaka, Jakarta.

Zulfikar. 2013. Manajemen Pemeliharaan Ayam Petelur Ras. Tesis. Pasca Sarjana Kesehatan Masyarakat Veteriner (Kesmavet) Universitas Syiah Kuala, Banda Aceh




DOI: https://doi.org/10.21157/j.med.vet..v11i1.3986

DOI (PDF): https://doi.org/10.21157/j.med.vet..v11i1.3986.g6918

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