10. Isolation and Identification of Lactic Acid Bacteria Genus Pediococcus from Sumatran Orangutan (Pongo abelii) faeces at Kandi Zoo and Kinantan Zoo West Sumatera

Resti Reimena, Erina Erina, Darniati Darniati, Fakhrurrazi Fakhrurrazi, Darmawi Darmawi, Hamdani Budiman

Abstract


This research has purposes to isolating and identifying the lactic acid bacteria genus Pediococcus in sumatran orangutan (Pongo abelii) feaces. Feaces are isolated from one orangutan at Kandi Zoo and two orangutans at Kinantan Zoo. The isolating of the lactic acid bacteria was done by using streak plate method with the MRSA (de Man Rogosa Sharpe Agar) specific media. Identification was done by the morphological observing, Gram stain, catalase test, oxidase test and biochemical test. The result showed that one isolate feaces from Kandi Zoo has cocci shaped with the isolate prediction approaches genus Pediococcus sp. The characteristic features of isolate are cocci shaped, Gram positive, tetrad cell structure, nonmotile, catalase negative, and oxidase negative. Based on these characteristics, it can be concluded that there is a lactic acid bacteria coloni which grouped into genus Pediococcus in male orangutan feaces from Kandi Zoo

Keywords


sumatran orangutan; lactic acid bacteria; Pediococcus.

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References


Aieolo, E.S. 2000. The Merck Veterinary Manual. 8th Ed. Merck and Co, Inc. USA.

Brown, A. 2001. Microbiological Application Lab Manual. 8th Ed. The McGraw-Hill Companies, New York.

Colome, J.S. 2001. Laboratory Exercises in Microbiology. West Publishing Company, New York.

Commane, D., R. Hughes, C. Shortt, and I. Rowland. 2005. The potential mechanisms involved in the anti-carcinogenic action of probiotics. Mutation Research 591: 276-289 (Abstract).

Cowan, S.T. 1975. Manual for Identification of Medical Bacteria. 2nd Ed. Cambridge University Press, Cambridge.

Damayanti, E., A.E. Suryani, A. Sofyan, M.F. Karimy, dan H. Julendra. 2015. Seleksi bakteri asam laktat dengan aktivitas anti jamur yang diisolasi dari silase dan saluran cerna ternak. Agritech. 35(2): 11-16.

Fardiaz, S. 2002. Mikrobiologi Pangan 1. PT Gramedia Pustaka Utama, Jakarta.

Fitri, L. dan Y. Yasmin. 2011. Isolasi dan pengamatan morfologi koloni bakteri kitinolotik. Jurnal Ilmiah Pendidikan Biologi. 3(2): 20-25.

Frazier, W.C. and D.C. Westhof. 1988. Food Microbiology. McGraw Hill Book Company, Singapore.

Gill, H.S. and F. Guarner. 2004. Probiotics and human health: A clinical prespective. Postgrad Med. J. 80: 516-526.

Ginting, Y.W.S.B. 2006. Studi Reintroduksi Orangutan Sumatera (Pongo pygmaeus abelii Lesson, 1827) yang Dikembangkan di Stasiun Karantina Medan dan Stasiun Karantina Reintroduksi Jambi. Skripsi. Fakultas Kehutanan Institut Pertanian Bogor. Bogor.

Harris, R.S. dan E. Karmas. 1989. Evaluasi Gizi pada Pengolahan Bahan Pangan. Institut Teknologi Bandung Press, Bandung.

Lay, B.W. 1994. Analisis Mikroba di Laboratorium. PT. Raja Grafindo Persada, Jakarta.

Minarwanto, H. 2008. Studi Aktivitas Harian Orangutan (Pongo pygmaeus wurmbii, Groves 2001) di Orangutan Care Center and Quarantine Pangkalan Bun, Kalimantan Tengah. Skripsi. Fakultas Kehutanan Institut Pertanian Bogor. Bogor.

Mutmainnah, H. 2008. Isolasi dan Karakterisasi Bakteri Probiotik Dari Saluran Pencernaan Ayam Kampung (Gallus domesticus). Skripsi. Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Hasanuddin. Makassar.

Osmanagaoglu, O., F. Kiran, and I.F. Nes. 2011. A Probiotic Bacterium, Pediococcus pentosaceus OZF Isolated from Human Breast Milk Produces Pediocin AcH/PA-1. African Journal of Biotechnology. 10(11): 2070-2079.

Prasthani, I.P.H. 2012. Isolasi dan Karakterisasi Bakteri Asam Laktat Asal Feses Orangutan (Pongo pygmaeus) Sebagai Kandidat Probiotik. Skripsi. Fakultas Kedokteran Hewan Universitas Brawijaya. Malang.

Purwani, E., S.W.N. Hapsari, dan R. Rauf. 2009. Respon hambatan bakteri Gram positif dan negatif pada ikan nila (Oreochromis niloticus) yang diawetkan dengan ekstrak jahe (Zingiber officinale). Jurnal Kesehatan. 2(1): 61-70.

Schrezenmeir, J.M. and D. Vrese. 2008. Probiotics, prebiotics, and synbiotics. Adv Biochem Engin/Biotechnol. 111: 1-66.

Septiarini, W.E. 2012. Aktivitas Antimikroba Bakteri Asam Laktat (BAL) yang diisolasi dari Feses Orangutan (Pongo pygmaeus) terhadap Penghambatan Pertumbuhan Bakteri Enterik Patogen secara In Vitro. Skripsi. Program Kedokteran Hewan Universitas Brawijaya. Malang.

Soomro, A.H., T. Masud, and K. Anwaar. 2002. Role of lactic acid bacteria (LAB) in food preservation and human health a review. Pakistan Journal of Nutrition. 1(1):20-24.

Sudiarta, I.W. 2012. Isolasi dan Identifikasi Bakteri Asam Laktat Indigenous dari Kecap Ikan Lemuru (Sardinella longiceps) Selama Fermentasi. Tesis. Program Pascasarjana Universitas Udayana. Denpasar.

Surono, I.S. 2004. Probiotik Susu Fermentasi dan Kesehatan. Tri Cipta Karya, Jakarta

Syahrurachman. 1994. Buku Ajar Mikrobiologi Kedokteran. Edisi Revisi. Bina Rupa Aksara, Jakarta.

Suhandi, A.P., D. Yoza, dan T. Arlita. 2015. Perilaku harian orangutan (Pongo pygmaeus Linnaeus) dalam konservasi ex-situ di kebun binatang Kasang Kulim kecamatan Siak Hulu kabupaten Kampar Riau. Jom. Faperta. 2(1): 1-5.

Utami, D.A. 2011. Karakterisasi Molekular Bakteri Asam Laktat (BAL) Probiotik dengan Gen 16s rRNA yang Berpotensi Menghasilkan Bakteriosin dari Fermentasi Sirsak (Annona maricata. L) di Sumatera Barat. Tesis. Program Pascasarjana Universitas Andalas. Padang.

Wikandari, P.R., Suparmo, Y. Marsono, dan E.S. Rahayu. 2012. Karakterisasi bakteri asam laktat proteolitik pada bekasam. Jurnal Natur Indonesia 14(2) : 120-125.




DOI: https://doi.org/10.21157/j.med.vet..v11i1.3524

DOI (PDF): https://doi.org/10.21157/j.med.vet..v11i1.3524.g6950

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