MUCOLYTIC ACTIVITY OF ROSELLE (Hibiscus sabdariffa L.) CALYCES EXTRACT ON COW INTESTINAL MUCUS

Nerdy Nerdy, Kesaktian Manurung

Abstract


This study aimed to examine the mucolytic activity of roselle calyces extract on cow intestinal mucus in vitro. The extraction of roselle
calyces was performed by the subsequence maceration method using different polarity solvent (n-hexane, ethyl acetate, and methanol). Mucolytic
activity of each extract was evaluated by measuring the viscosity of mixture of cow intestinal mucus and phosphate buffer pH 7 compared to
acetylcysteine as the standard mucolytic drug. The result showed that the decreasing of cow intestinal mucus and phosphate buffer pH 7 mixtures
viscosity was indicated the increasing of mucolytic activity. The n-hexane, ethyl acetate, and methanol extract of roselle calyces exhibit dosedependent
mucolytic activity. Mucolytic activity of roselle calyces ethyl acetate extract with dose of 0.8% and roselle calyces methanol extract
with dose of 0.6% showed no significant difference to mucolytic activity from acetylcysteine with dose of 0.1% (positive control). An increased
dose of roselle calyces extract will decrease the viscosity of cow intestinal mucus and phosphate buffer pH 7 mixtures, indicating an increase in
mucolytic activity.


Keywords


cow intestinal; mucolytic; roselle calyces; mucus; viscosity

Full Text:

PDF

References


Abubakar, M.G., A.N. Ukwuani, U.U. Mande. 2015. Antihypertensive Activity of Hibiscus sabdariffa aqueous calyx extract in Albino Rats. Sky J. Biochem. Res. 4 (3): 16-20.

Adesina, S.K., I.I. Johnny, and G. Olayiwola. 2017. Plants in Respiratory Disorders II Antitussives. Br. J. Pharm. Res. 16 (3): 1-21.

Ali, M.K., A. Ashraf, N.N. Biswas, U.K. Karmakar, and S. Afroz. 2011. Antinociceptive, Antiinflammatory, and Antidiarrheal Activities of Ethanolic Calyx Extract of Hibiscus sabdariffa Linn. (Malvaceae) in Mice. Chin. J. Integr. Med. 9 (6): 626-631.

Altemimi, A., N. Lakhssassi, A. Baharlouei, D.G. Watson, and D.A. Lightfoot. 2017. Phytochemicals: Extraction, Isolation, and Identification of Bioactive Compounds from Plant Extracts. Plants. 6 (42): 1-23.

De Blasio, F., J.C. Virchow, M. Polverino, A. Zanasi, P.K. Behrakis, G. Kilinç, R. Balsamo, G. De Danieli, and L. Lanata. 2011. Cough Management Practical Approach. Cough. 7 (7): 1-12.

Ezzat, S.M., M.M. Salama, S.H.S. El-Din, S. Saleh, N.M. El-Lakkany, O.A. Hammam, M.B. Salem, and S.S. Botros. 2016. Metabolic Profile and Hepatoprotective Activity of the Anthocyanin Rich Extract of Hibiscus sabdariffa Calyces. Pharm. Biol. 54 (12): 3172-3181

Fahy, J.V., and B.F. Dickey. 2010. Airway Mucus Function and Dysfunction. N. Engl. J. Med. 363 (23): 2233-2247.

Jacquot, J., A. Hayem, C. Galabert. 1992. Functions of Proteins and Lipids in Airway Secretions. Eur. Respir. 5: 343-358.

Janson, C. 2017. Cough Preparations to Give or Not. Chron. Pharm. Sci. 1 (5): 262-267.

Kadri Z.H.M., A.H. Ad'hiah, and E.K. Shubber. 2013. Some Immunological Effects of Roselle Hibiscus sabdariffa L. Calyces in Albino Mice. J. Genet. Environ. Resour. Conserv. 1 (1): 50-55.

Keller, J.A., A.E. McGovern, and S.B. Mazzone. 2017. Translating Cough Mechanisms Into Better Cough Suppressants. J. Chest. 152 (4):833-841.

Lusida, T.T.E, B. Hermanto, and Sudarno. 2017. Antibacterial Effect of Roselle (Hibiscus sabdariffa) Extract against Staphylococcus epidermidis In Vitro. Id. J. Trop. Inf. Dis. 6 (4): 88-91.

Muctahara, N., N. Cahyani, H. Febriani, M. Isrul, and W.O. Yuliastri. 2017. In Vitro Study of Mucolytic Activity of Extract and Essential Oil of Citrus Maxima Peel. Res. J. Pharm. Biol. Chem. Sci. 8 (6): 112-116.

Nerdy. 2017. In Silico Docking Roselle (Hibiscus sabdariffa l.) Calyces Flavonoids as Antimalarial against Plasmepsin 1 and Plasmepsin 2. Asian J. Pharm. Clin. Res. 10 (10): 183-186.

Obouayeba, A.P., S. Meité, L. Boyvin, D. Yeo, T.H. Kouakou, and J.D. N’Guessan. 2015. Cardioprotective and Antiinflammatory Activities of a Polyphenols Enriched Extract of Hibiscus sabdariffa Petal Extracts in Wistar Rats. J. Pharmacogn. Phytochem. 4 (1): 57-63

Pelaseyed, T., J.H. Bergström, J.K. Gustafsson, A. Ermund, G.M.H. Birchenough, A. Schütte, S. van der Post, F. Svensson, A.M. Rodríguez-Piñeiro, E.E.L. Nyström, C. Wising, M.E.V. Johansson, and G.C. Hansson. 2014. Mucus and Mucins of the Goblet Cells and Enterocytes Provide the First Defense Line of the Gastrointestinal Tract and Interact with the Immune System. Immunol. Rev. 260 (1): 8-20.

Prabhakaran, D., M.M. Senthamilselvi, and A. Rajeshkanna. 2017. Anticancer Activity of Hibiscus sabdariffa.L (Flowers) against Human Liver Cancer (Hepg2) Cell Line. Int. J. ChemTech Res. 10 (12): 351-356.

Rosemary, Rosidah, and G. Haro. 2014. Antidiabetic Effect of Roselle Calyces Extract (Hibiscus sabdariffa L.) in Streptozotocin Induced Mice. Int. J. PharmTech Res. 6 (5): 1703-1711.

Weinberger, M., and B. Lockshin. 2017. When is Cough Functional, and How should it be Treated. Breathe. 13: 22-30.




DOI: https://doi.org/10.21157/j.ked.hewan.v12i2.9621

Refbacks

  • There are currently no refbacks.


Copyright (c) 2018 by author and J. Ked. Hewan

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Editorial Office

Jurnal Kedokteran Hewan (Indonesian Journal of Veterinary Sciences)

Faculty of Veterinary Medicine Syiah Kuala University
Jl. Tgk. Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah Darussalam
Banda Aceh 23111, Indonesia
Phone/Fax. +62-651-7551536, CP: +6285260153935; +6281362693330
e-mail: jkh@unsyiah.ac.id; jurnal_khusk@yahoo. 

  

p-ISSN: 1978-225X e-ISSN: 2502-5600


Creative Commons License
J Ked Hewan
is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.