THE ROLE OF GUAVA FRUIT FILTRATE ON MAINTAINING THE PRE-FREEZING AND POST-THAWED QUALITY OF BALI BULL SPERMATOZOA

I Wayan Lanus Sumadiasa, Lukman Lukman, Rodiah Rodiah

Abstract


This study aimed to determine the role of guava fruit filtrate (GFF) on maintaining the pre-freezing and post-thawed quality of Bali bull
spermatozoa in tris-egg yolk (TEY) extender. The bovine semen was collected using artificial vagina twice a week (n= 10). Ejaculate samples
were divided into four tubes and each tube was respectively added the following diluents: TEY, TEY + 5% GFF, TEY + 10% GFF, and TEY +
15% GFF. The diluted semen was filled into the straws (0.25 mL) and cooled in refrigerator at 4° C for 2.5 hours. The samples were kept on
vapor phase of liquid nitrogen (N2) (-120° C) for 10 minutes prior to be stored in liquid phase (-196° C). The quality of pre-freezing and postthawed
spermatozoa in straw sample (i.e. motility, viability, and abnormality) was evaluated under light microscopy at 400x magnification. Data
were analysed statistically. The percentage of motility and viability both on pre-freezing and post-thawed process were significantly higher in
semen diluted with TEY + 10% GFF compared to control (TEY), TEY + 5% GFF, and TEY + 15% GFF. Addition of 10% GFF into tris-egg yolk
extender play role for maintaining the quality of pre-freezing and post-thawed Bali bull spermatozoa.


Keywords


bovine; guava filtrate; pre-freezing and post-thawed; spermatozoa

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References


Abavisani, A., J. Arshami, A.A. Naserian, M.A.S. Kandelousi and M. Azizzadeh. 2013. Quality of bovine chilled or frozen-thawed semen after addition of omega-3 fatty acids supplementation to extender. Int J Fertil Steril 7(3) : 161 – 168.

Akhter, S., B.A. Rakha, S.M.H. Andrabi, M.S. Ansari. 2010. Comparison of egg yolks from three avian species in extender for cryopreservation of Sahiwal bull epididymal spermatozoa. Animal Science Papers and Reports 29(2):131 – 138.

Andrabi, S.M.H., S. Naheed, L.A. Khan and N. Ullah. 2002. Semen characteristics of crossbreed (Friesian x Sahiwal) bulls at livestock research station, national agricultural research centre, Islamabad. Pakistan Vet.J. 22(4) : 181 – 187.

Andrabi, S. M.H., M.S. Ansari, N. Ullah and M. Afzal. 2008. Effect of non-enzymatic antioxidants in extender on post-thaw quality of buffalo (Bubalus Bubalis) bull spermatozoa. Pakistan Vet. J., 28(4): 159-162.

Asadpour, R., Razi Jafari and Hossein Tayefi-Nasrabadi. 2011. Iinfluence of added vitamin C and vitamin E on frozen-thawed bovine sperm cryopreserved in citrate and tris-base extenders. Veterinary Reseacrh Forum, 2(1) : 37 – 44.

Bansal, A.K. and G. S. Bilaspuri. 2011. Impacts of oxidative stress and antioxidants on sperma functions : Review Article. SAGE-Hindawi Access to Research. Veterinary Medicine International (2011) : 1 – 7.

Chanapiwat, P., K. Kaeoket and P. Tummaruk. 2011. Cryopreservation of Boar Sperma by Egg Yolk-Based Extenders Containing Lactose or Fructose is Better Than Sorbitol. Theriogenology. J. Vet. Med. Sci.74(3): 351 – 354.

Delgado, P.A., T.D. Lester and R.W. Rorie. 2009. Effect of low-sodium, choline-based diluents on viability of bovine sperm storred at refrigerator temperatures. AAES Research Series 574 : 77 – 79.

Funahashi, H. and T. Sano. 2005. Select antioxidants improve the function of extended boar sperma stored at 10°C. Theriogenology 63(6) : 1605 – 1616.

Garner, D.L. and E.S.E. Hafez. 2008. Spermatozoa and seminal plasma. In : Reproduction in Farm Animals. Hafez, B. and E.S.E. Hafez (Eds.) , 7th ed. Blackwell Publishing : 97–109.

Gillan, L., W.M.C. Maxwell and G. Evans. 2004. Preservation and evaluation of sperma for artificial insemination. Reproduction, Fertility and Development : 447 – 452.

Hossain, M.E., M.M. Khatun, M.M. Islam and O.F. Miazi. 2012. Semen characteristics of breeding bulls at the Central Cattle Breeding and Dairy Farm of Bangladesh. Bang. J. Anim. Sci. 41(1) : 1 – 5.

Hui-Yin, C. and Y. Gow-Chin. 2006. Antioxidant activity and free radical-scavenging capacity of extracts from guava (Psidium guajava L.) leaves. J.foodchem. 02(047) : 686 – 694.

Ibrahim, S.F., K. Osman, S. Das, A.M. Othman, N.A. Majid and M.P.Ab. Rahman. 2008. A Study of the Antioxidant Effect of Alpha Lipoic Acids on Sperm Quality. Clinics (Sao Paulo) 63(4): 545 – 550.

Kaeoket, K., P. Chanapai, P. Junchiyaphoom and P. Chanapiwat. 2011. The effect of using long term and short term extenders during cooling process on the quality of frozen boar sperma. Thai J Vet Med. 41(3): 283 – 288.

Kothari, S., A. Thomson, A. Agarwal and S.S. du Plessis. 2010. Free radical : Their beneficial and detrimental effects on sperm function. Review artice : Indian Journal of Experimental Biology, (48) : 425 – 435.

Kulaksiz, R. and A. Daskin. 2010. In vitro evaluation of Saanen buck sperma frozen in different extenders supplemented with various antioxidants. Ankara Üniv Vet Fak Derg (57) : 151 – 156.

Leahy, T. and S.P. de Graaf. 2012. Seminal Plasma and its Effect on Ruminant Spermatozoa During Processing. Reprod. Dom. Anim. 47(Suppl.4): 207-213.

Lobo, V., A. Patil, A. Phatak and N. Chandra. 2010. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010 Jul-Dec; 4(8): 118–126.

Mittal, P., Vikas Gupta, Gurpreet Kaur, Ashish K. Garg and Amarjeet Singh. 2010. Phytochemistry and pharmacological activities of Psidium guajava : A. Review. IJPSR, 1 (9) : 9 – 19.

Norshazila, S., S.I. Zahir, K.M. Suleiman, MR. Aisyah and K.K. Rahim. 2010. Antioxidant Levels and Activities of Selected Seeds of Malaysian Tropical Fruits. Mal J Nutr 16(1): 149-159.

Patel, B.R. and G.M. Siddiquee. 2013. Physical and morphological characteristics of Kankrej bull semen. Vetworld. 6(7) : 405 – 408.

Rehman, Z.U., M.U. Samo, T.A. Qureshi, S. Khan, M.S. Qureshi, F.A. Khan and S. Bahadadr. 2012. Studies on the freezability of Kundhi Buffalo bull semen. Journal of Animal and Plant Sciences, 22(2 Suppl.) : 18 – 23.

Rahmat, A., M.F.A. Bakar and Z. Hambali. 2006. The effeccts of guava (Psidium guajava) consumption on total antioxidant and lipid profile in normal male youth. AJFAND, Formerly AJFNS 6(2) : 1 – 12.

Rao, A.V. and L.G.Rao. 2007. Carotenoids and human health. Invited review. Elsevier. Pharmacological Research (55) : 207-216.

Sarder, M.J.U. 2007. Environment related variations in the semen characteristics of bulls used for Artificial Insemination (AI) programme in Bangladesh. Univ. j. zool. Rajshahi Univ. (26) : 81-88.

Stevanov, R., M. Sabev and M. Ivanova-Kicheva. 2011. Protective effect of the antioxidant enzyme on sperm from Bubalus bubalis type after cryoconservation. Biotechnology in animal husbandry 27(3) : 934 – 949.

Sumadiasa, IW.L. 2015. Substitusi kuning telur di dalam pengencer CEP-2 terhadap kualitas dan fertilitas spermatozoa sapi Bali selama simpan dingin. Disertasi. Universitas Brawijaya. Malang.

Sumadiasa, IW.L., T. Susilawati, G. Ciptadi and N. Isnaini. 2015. The potency of guava filtrate (Psidium guajava Linn) for preservation of Bali bull spermatozoa. IOSR-JAVS, 8(Issue 5 Ver. I) : 51-57.

Tavilani, H., M.T. Goodarzi, A. Vaisi-raygani, S. Salimi and T. Hassanzadeh. 2008. Activity of antioxidant enzymes in semnal plasma and their relationship with lipid peroxidation of spermatozoa. International Braz J. Urol. 34(4) : 485 – 491.

Thaipong, K., U. Boonprakob, L. Cisneros-Zevallos and D. H. Birne. 2005. Hydrophilic and lipophilic antioxidant activities of guava fruits. Shoutheast Asian J trop Med Public Health 36(suppl 4) : 254 – 257.

Toelihere, M.R. 1993. Inseminasi buatan pada ternak. Penerbit Angkasa Bandung.

Trout, S.W. 2012. Evaluation of Different Concentrations of Egg Yolk in Canine Frozen Semen Extender. Thesis. Biomedical and Veterinary Sciences, Faculty of the Virginia Polytechnic, Institute and State University : 1 – 50.

Vishwanath, R. and P. Shannon. 2000. Storage of bovine sperma in liquid and frozen state. Animal Reproduction Science 62: 23–53.

Yavas, T.K. and A. Daskin. 2012. Effect of alternative cryopreservation procedures on bull sperma. Short Communication / Kısa Bilimsel Çalışma. Ankara Üniv Vet Fak Derg, 59 : 231 – 234.

Zeitoun, M.M. and M.A. Al-Damegh. 2015. Effect of nonenzymatic antioxidants on sperm motility and survival relative to free radicals and antioxidant enzymes of chilled-stored ram semen. Open Journal of Animal Sciences, (5) : 50–58.




DOI: https://doi.org/10.21157/j.ked.hewan.v12i2.8801

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