MOLECULAR DETECTION OF GENE RESISTANT TO VARIOUS INSECTICIDES IN Aedes aegypti AT BANYUWANGI EAST JAVA USING POLYMERASE CHAIN REACTION

Aditya Yudhana, Ratih Novita Praja, Maya Nurwartanti Yunita

Abstract


The purpose of this study was to determine the type of insecticides which Aedes aegypti mosquitoes are resistant to, so that other susceptible
insecticides still can be used. The study was a cross sectional epidemiological study with cluster sampling in Sub-district Wongsorejo,
Banyuwangi, Muncar, Tegaldlimo, Kalibaru which is considered sufficient to represent Banyuwangi Regency. Mosquito samples appropriate to
the characteristics were isolated and tested for insecticide-resistance primer namely voltage gated sodium channel (VGSC) to determine the
specific resistance expressed in mosquitoes. The result of resistance test using WHO standard method showed that Aedes aegypti mosquito from
Banyuwangi Regency were resistant to cypermethrin 0.25% and malathion 0.8%. There was a VGSC coding gene with 250 bp band detected
using polymerase chain reaction (PCR) technique which was associated with the resistance of Aedes aegypti mosquitoes in Banyuwangi Regency
to organophosphate insecticides (malathione) and pyrethroid insecticides (cypermethrin).


Keywords


Aedes aegypti; insecticide; polymerase chain reaction; resistance

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DOI: https://doi.org/10.21157/j.ked.hewan.v12i1.7625

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