COMPARISON OF POLYPEPTIDE PROFILE OF Trypanosoma evansi ISOLATES FROM INDONESIA AND THEIR RELATION TO BIOTYPE AND SENSITIVITY TO TRYPANOCIDAL

Ichwan Yuniarto, Didik T Subekti, Umi Cahyaningsih, Fadjar Satrija

Abstract


This study aimed to determine whether the variant or biotype of Trypanosoma evansi can be seen from their polypeptide profiles using 12%
sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) stained with Brilliant Blue Commasie. The results generally showed that
the molecular weight (MW) of polypeptides from nine isolates from East Java, Central Java, Banten, South Kalimantan, Central Kalimantan, and
Lampung provinces were in the range of 85.46 to 15.76 kD and each isolate has different polypeptide profile. Isolates A13 and A14 were isolated
from the same place but have different polypeptide profiles. Likewise, isolates S13 and S18 also have different polypeptide profiles despite being
isolated from the same place at the same time. On the other hand, isolate 372, 87, and 06 have different protein profiles but was classified in the
same biotype namely biotype I. Generally, the difference in protein profile actually more related to the biological diversity of the metabolism of
each Trypanosoma evansi isolate from Indonesia.


Keywords


biotype; protein profile; SDS PAGE; surra; Trypanosoma evansi

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DOI: https://doi.org/10.21157/j.ked.hewan.v12i2.11486

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