Cut Nila Thasmi, Tongku Nizwan Siregar, Sri Wahyuni, Dwinna Aliza, Hamdan Hamdan, Budianto Panjaitan, Nuzul Asmilia, Husnurrijal Husnurrijal


The objective of this research was to determine steroid level of repeat breeding Aceh cattle. This research included 17 female Aceh cattle, aged 3-5 years, who had been pregnant at least once. The cows were divided into two groups, fertile cows (n=7) and repeated breeding cows (n=10). All cows were estrous synchronized by 5 ml PGF2α intramuscularly (LutalyseTM, Pharmacia & Upjohn Company, Pfizer Inc.) via double injection pattern, with 11 days interval. Blood collection for progesterone measurement began on the day of the last PGF2α injection, while the blood sample for estradiol was collected during standing heat. Steroid analysis was performed by enzyme linked immunosorbent assay method (ELISA). Injection of the second PGF2α in both treatment groups managed to induce estrus in 5 cows (71.4%) in fertile cow group, and 9 cows (90.0%) in repeat breeding cow group. Progesterone level showed significant difference (P>0.05) on Day 5 after PGF2α injection, with 2.88±2.14 ng/ml (fertile cows) and 1.13±0.68 ng/ml (repeat breeding cows). Repeat breeding Aceh cattle have lower progesterone level on Day 5 after PGF2α injection compared to fertile cows.

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