Sarah Firdausa, Maung Maung Cho, Khin Maung Maung, Nilar Aung, Norsidah Kuzaifah


Diabetes is a leading cause of cellular oxidative stress which can lead to toxic effects on structure and function of organs, including β-cell in the pancreas. Islet cells of pancreas are among the tissues that have the lowest level of an antioxidant defense. In a chronic hyperglycemic state, a number of reactive oxygen species accumulate in an excess amount and cause chronic oxidative stress in the islet cell. This study aimed to investigate the effect of Tinospora crispa aqueous extract (TC) on oxidant status in streptozotocin (STZ)-induced diabetic rats. Fifty mg/kgbw of STZ was intraperitoneally induced to Sprague-Dawley rats. The animals were divided into five groups (n=6); normal (N), normal TC treated (NT), diabetic (D), diabetic TC treated (DT), and diabetic treated with Vitamin E (DE), respectively. Treatment was delivered for 4 weeks and blood sample were withdrawn for parameters measurement. The data among groups were compared using one way ANOVA test, while paired t-test was used to analyse the differences between data of pre and post treatment. In normal groups, the antioxidant and oxidative stress level of N group did not differ noticeably with NT group. In diabetic groups, the antioxidant status in DT rats showed that TC might prevent a decrease of superoxide dismutase level which was reduced in other diabetic groups. It also increased the glutathione peroxidase enzymes in animals TC treated (NT and DT). Oxidative stress level (lipid hydroperoxide and isoprostane) went up substantially in group D and no significant changes in other groups. These results showed that TC might protect diabetic subjects from reactive oxygen species generation by enhanced ant-oxidant enzymes level.

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